The histidine-phosphocarrier protein of the phosphoenolpyruvate: sugar phosphotransferase system of Bacillus sphaericus self-associates

Autor: Claudio N. Cavasotto, Ana Isabel Díez-Peña, José García de la Torre, Julio Bacarizo, Ana Cámara-Artigas, José G. Hernández-Cifre, Sergio Martínez-Rodríguez, Adrián Velázquez-Campoy, José L. Neira, Rosa Doménech
Přispěvatelé: Ministerio de Ciencia e Innovación (España), European Commission, Diputación General de Aragón, Junta de Andalucía, Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Instituto de Biocomputación y Física de Sistemas Complejos (España)
Rok vydání: 2013
Předmět:
Models
Molecular
Proteomics
BACILUS SPHAERICUS
Protein Denaturation
Protein Folding
Hot Temperature
lcsh:Medicine
Bacillus
Plasma protein binding
Bacillus sphaericus
Biochemistry
Protein Structure
Secondary
purl.org/becyt/ford/1 [https]
Protein structure
ENZYME I
Macromolecular Structure Analysis
Phosphorylation
Biomacromolecule-Ligand Interactions
lcsh:Science
Multidisciplinary
biology
Physics
Streptomyces coelicolor
PEP group translocation
Bioquímica y Biología Molecular
Hydrogen-Ion Concentration
PROTEIN-PROTEIN INTERACTION
Thermodynamics
Phosphoenolpyruvate carboxykinase
CIENCIAS NATURALES Y EXACTAS
Protein Binding
Research Article
Protein Structure
Biophysics
Phosphocarrier protein
macromolecular substances
Calorimetry
Protein Chemistry
Ciencias Biológicas
Bacterial Proteins
Histidine
purl.org/becyt/ford/1.6 [https]
Phosphoenolpyruvate Sugar Phosphotransferase System
Protein Interactions
Biology
lcsh:R
fungi
Proteins
Computational Biology
biology.organism_classification
carbohydrates (lipids)
Spectrometry
Fluorescence
HISTIDINE-PHOSPHOCARRIER PROTEIN
biology.protein
Hydrodynamics
bacteria
lcsh:Q
Peptides
Zdroj: PLoS ONE
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE, Vol 8, Iss 7, p e69307 (2013)
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1932-6203
Popis: 15 pags, 7 figs, 1 tab
The phosphotransferase system (PTS) is involved in the use of carbon sources in bacteria. Bacillus sphaericus, a bacterium with the ability to produce insecticidal proteins, is unable to use hexoses and pentoses as the sole carbon source, but it has ptsHI genes encoding the two general proteins of the PTS: enzyme I (EI) and the histidine phosphocarrier (HPr). In this work, we describe the biophysical and structural properties of HPr from B. sphaericus, HPrbs, and its affinity towards EI of other species to find out whether there is inter-species binding. Conversely to what happens to other members of the HPr family, HPrbs forms several self-associated species. The conformational stability of the protein is low, and it unfolds irreversibly during heating. The protein binds to the N-terminal domain of EI from Streptomyces coelicolor, EINsc, with a higher affinity than that of the natural partner of EINsc, HPrsc. Modelling of the complex between EINsc and HPrbs suggests that binding occurs similarly to that observed in other HPr species. We discuss the functional implications of the oligomeric states of HPrbs for the glycolytic activity of B. sphaericus, as well as a strategy to inhibit binding between HPrsc and EINsc. © 2013 Doménech et al.
This work was supported by the Spanish Ministerio de Ciencia e Innovación (MCINN) (CTQ2011-24393, and CSD2008-00005 to JLN; BIO2009-13261-C02- 01/02 and P09-CVI-5063, with Fondo Social Europeo (ESF) to ACA; and BFU2010-19451 to AVC), Diputación General de Aragón (PI044/09 to AVC), intramural BIFI 2011 projects (to AVC and JLN), Junta de Andalucía (BIO-328 to ACA), and by grants from the Agencia Nacional de Promoción Científica y Tecnológica Argentina (ANPCyT; PICT-2011-2778) (to CNC). SMR was supported by the CSD2008-00005. The stays of RD in the laboratory of AVC were supported by the Spanish Ministerio de Ciencia e Innovación (BFU2008-02302-BMC).
Databáze: OpenAIRE
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