Caspase-3-related apoptosis prevents pathological regeneration in a living liver donor rat model
| Autor: | Daniel Hernandez-Baltazar, Laura Mireya Zavala-Flores, Rasajna Nadella, Arnulfo Villanueva-Olivo, Paola Belem Pensado-Guevara, Aurora Sánchez-García, Abraham Puga-Olguín, Abril Alondra Barrientos-Bonilla |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_treatment Cell Caspase 3 Apoptosis Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactate dehydrogenase medicine Living Donors Animals Hepatectomy Lobules of liver 030212 general & internal medicine Rats Wistar Cell Proliferation business.industry General Medicine Cell counting Liver Regeneration Rats medicine.anatomical_structure chemistry Liver 030220 oncology & carcinogenesis business Immunostaining |
| Zdroj: | Advances in medical sciences. 66(1) |
| ISSN: | 1898-4002 |
| Popis: | Purpose The main goal of this study was to determine the relationship of cleaved-caspase-3 (C3)-related apoptosis and hepatic proliferation, during the liver repopulation in a living liver donor rat model. Material/methods Thirty-three animals were randomized into eleven groups and evaluated on postoperative from 3 h until 384 h after 30%-partial hepatectomy (30%-PHx). Liver sections (5 μm) were processed by hematoxylin-eosin, and immunostaining for C3, accompanied by hepatic function test. C3 content and the hepatic lobule enlargement were analyzed by optical density, followed by cell counting. Results Transient variations of alanine transferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were found. Significant increase in the C3 levels, and cell nuclei number, were detected at 12 h and 48 h after 30%-PHx, evidencing a correlation of p = −0.3679. Conclusion In the 30%-PHx rat model, C3-related apoptosis prevents proliferative pathological conditions during the hepatic lobule re-modeling. |
| Databáze: | OpenAIRE |
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