Osteopontin is a constitutive component of normal elastic fibers in human skin and aorta
| Autor: | M. Baccarani-Contri, F. Taparelli, I. Pasquali-Ronchetti |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Mineralized tissues Aging Pathology medicine.medical_specialty Adolescent Sialoglycoproteins Immunocytochemistry Human skin Epitope Fetus stomatognathic system Reference Values medicine.artery medicine Humans Osteopontin Child Molecular Biology Aorta Aged Skin Kidney biology Chemistry Infant Newborn Middle Aged Elastic Tissue Immunohistochemistry medicine.anatomical_structure biology.protein Elastic fiber |
| Zdroj: | Matrix Biology. 14:553-560 |
| ISSN: | 0945-053X |
| DOI: | 10.1016/s0945-053x(05)80004-6 |
| Popis: | Osteopontin is an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels; its biological role is still largely undefined. In the present study, immunocytochemical approaches showed that osteopontin is localized within normal elastic fibers of human skin and aorta. Antibodies raised against human bone osteopontin (LF7) or against human osteopontin synthetic peptide (amino acids 1–10, LF19) recognized epitopes associated with the amorphous material within the elastic fibers. Elastic fiber-associated micro-fibrils were always negative. The positivity for osteopontin of the elastic fibers was independent of age and could be observed in fetal skin and aorta as well as in the same of children, young adults and old subjects. The altered elastic fibers in the skin of old individuals were only fairly positive for osteopontin. The presence of osteopontin within the elastic fibers suggests that it may play a role against the observed tendency of elastic fibers to favor mineral precipitation. A role of osteopontin in modulating crystal nucleation and growth in mineralizing tissues and, more generally, in conditions in which mineral precipitation should be controlled is also possible. |
| Databáze: | OpenAIRE |
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