Plasmonic targeting of cancer cells in a three-dimensional natural hydrogel
| Autor: | Matan Hamra, Limor Minai, Dvir Yelin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Cell Metal Nanoparticles Nanoparticle 02 engineering and technology 03 medical and health sciences Cell Line Tumor medicine Humans General Materials Science Irradiation Cell damage Plasmon Lasers Hydrogels Fibroblasts 021001 nanoscience & nanotechnology medicine.disease 030104 developmental biology medicine.anatomical_structure Colloidal gold Cell culture Cancer cell Biophysics Gold 0210 nano-technology Nanospheres |
| Zdroj: | Nanoscale. 10:17807-17813 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | Using specifically designed gold nanoparticles and local laser irradiation, individual cells and small cell clusters could be targeted on a microscopic scale with minimal toxicity to nearby tissue. To date, most scientific studies and technological demonstrations of this approach were conducted on two-dimensional cultures, while most feasibility tests and preclinical trials were conducted using animal models. For bridging the gap between two-dimensional cell cultures and animal experiments, we propose and demonstrate the use of a natural hydrogel for studying the effect of intense, ultrashort laser pulses on a gold nanoparticle targeted tissue. Using illumination parameters comparable to those used with two-dimensional cultures, we show the complete eradication of multilayered cell colonies comprising normal fibroblasts and malignant epithelial cells co-cultured on a hydrogel scaffold. By evaluating the extent of cell damage for various pulse durations at off-resonance irradiation, we find that the observed damage mechanism was dominated by rapid thermal transitions around the gold nanospheres, rather than by photoionization. The work provides a new tool for understanding the complex pulse-particle-tissue interactions and demonstrates the important role of nanoparticle mediated cavitation bubbles in a thick, multilayered tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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