Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models
| Autor: | Dong Kun Lee, Seong-Geun Hong, Sang Soo Kang, Hyun Joon Kim, Chang-Woon Kim, Nam-Gil Kim, Min Seok Woo, Jong Ryeal Hahm, Jung Hwan Kim, Yeung Joon Choi, Eun-Jin Kim, Eui-Jung Shin, Jaehee Han, Si-Hyang Park, Dawon Kang, Adrian S. Siregar, Jin-Mok Kim, Marie Merci Nyiramana |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Pharmaceutical Science Aldehyde dehydrogenase Hangovers Pharmacology medicine.disease_cause Article Gene Expression Regulation Enzymologic Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Drug Discovery medicine Animals Ethanol metabolism Crassostrea lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology Alcohol dehydrogenase Liver injury 0303 health sciences Ethanol biology alcohol Hydrolysis Dipeptides CYP2E1 Aldehyde Dehydrogenase medicine.disease Mice Inbred C57BL Oxidative Stress lcsh:Biology (General) chemistry inflammation biology.protein 030217 neurology & neurosurgery Oxidative stress liver injury |
| Zdroj: | Marine Drugs Marine Drugs, Vol 18, Iss 512, p 512 (2020) Volume 18 Issue 10 |
| ISSN: | 1660-3397 |
| Popis: | Accumulative alcohol hangovers cause liver damage through oxidative and inflammatory stress. Numerous antioxidant and anti-inflammatory reagents have been developed to reduce alcohol hangovers, but these reagents are still insignificant and have limitations in that they can cause liver toxicity. Oyster hydrolysate (OH), another reagent that has antioxidant and anti-inflammatory activity, is a product extracted through an enzymatic hydrolysis process from oysters (Crassostrea gigas), which can be easily eaten in meals. This study was aimed at determining the effects of OH on alcohol metabolism, using a single high dose of ethanol (EtOH) administered to rodents, by monitoring alcohol metabolic enzymes, oxidative stress signals, and inflammatory mediators. The effect of tyrosine-alanine (YA) peptide, a main component of OH, on EtOH metabolism was also identified. In vitro experiments showed that OH pretreatment inhibited EtOH-induced cell death, oxidative stress, and inflammation in liver cells and macrophages. In vivo experiments showed that OH and YA pre-administration increased alcohol dehydrogenase, aldehyde dehydrogenase, and catalase activity in EtOH binge treatment. In addition, OH pre-administration alleviated CYP2E1 activity, ROS production, apoptotic signals, and inflammatory mediators in liver tissues. These results showed that OH and YA enhanced EtOH metabolism and had a protective effect against acute alcohol liver damage. Our findings offer new insights into a single high dose of EtOH drinking and suggest that OH and YA could be used as potential marine functional foods to prevent acute alcohol-induced liver damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|