New Immunochromatographic Rapid Test for Diagnosis of Acute Puumala Virus Infection
Autor: | Vesa Koistinen, Pekka Eerikäinen, Ilkka Mononen, Ilpo Kuronen, Markku Parviainen, Antti Vaheri, Åke Lundkvist, Ale Närvänen, Helena Hujakka, Olli Vapalahti |
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Rok vydání: | 2001 |
Předmět: |
Microbiology (medical)
Orthohantavirus Immunodiffusion Time Factors Hantavirus Infections Fluorescent Antibody Technique Antibodies Viral Immunofluorescence Sensitivity and Specificity 03 medical and health sciences Virology medicine Humans Rheumatoid factor Nucleocapsid Antigens Viral 030304 developmental biology Immunoassay 0303 health sciences biology medicine.diagnostic_test 030306 microbiology Nucleocapsid Proteins biology.organism_classification Molecular biology 3. Good health Immunoglobulin M biology.protein Puumala virus Reagent Kits Diagnostic Bunyaviridae Antibody |
Zdroj: | Journal of Clinical Microbiology. 39:2146-2150 |
ISSN: | 1098-660X 0095-1137 |
Popis: | A new immunochromatographic rapid test, POC PUUMALA (Erilab Ltd., Kuopio, Finland), for detection of acute-phase Puumala virus (PUUV) infection was developed based on a highly purified baculovirus-expressed PUUV nucleocapsid protein antigen and lateral immunodiffusion techniques. After addition of sample (5 μl of serum, plasma, or fingertip blood) and buffer, PUUV-specific immunoglobulin M (IgM) antibodies, if present, together with the gold-conjugated anti-human IgM, formed a specific colored line in 5 min. The sensitivity and specificity of the test were evaluated with 200 serum samples and 30 fingertip blood samples. The reference method for the serum samples was a μ-capture enzyme immunoassay (EIA) for IgM and an immunofluorescence assay (IFA) for IgG antibodies. The analytical sensitivity and specificity of the rapid test were 100 and 99%, respectively, for unfrozen serum samples ( n = 103; 12 PUUV IgM-positive samples). When freeze-thawed serum samples were used, the sensitivity and specificity were each 97.1% ( n = 70; 35 PUUV IgM-positive samples). The specificity of the test was 96.2% for 27 serum samples with nonspecific IgM antibodies or rheumatoid factor (RF). The fingertip blood samples ( n = 30) were negative, but they gave clear positive results when spiked with IgM-positive sera ( n = 20). The results were in good agreement with the standard diagnostic methods. The rapid performance, the lack of need for refined laboratory equipment, and the high specificity with fresh serum and fingertip blood samples indicate that the developed POC PUUMALA rapid test is a useful tool for fast diagnosis of acute PUUV infection. |
Databáze: | OpenAIRE |
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