| Popis: |
Immunoblots of apoptotic proteins in docetaxel treated A2780 cells. A2780 cells were untreated or treated with 0.2 μM docetaxel for 24, 48, or 72 h. Protein lysates were prepared from the cells, resolved by SDS-PAGE, and transferred to polyvinylidene difluoride (PVDF) membranes. Immunoblotting was performed antibodies against full length caspase 3, cleaved caspase 3, Parp-1 and the loading controls GAPDH and β-actin. Primary antibodies for caspase-3 (3G2), PARP-1 (46D11) and GAPDH (14C10) were from Cell Signaling Technology, Inc. (New England Biolabs, Ltd., Whitby, ON, CA) while HRP-conjugated anti-mouse and –rabbit IgG secondary antibodies were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The Parp-1 antibody detected both full length and cleaved Parp-1. A. Immunoblot showing full length caspase 3. B. Immunoblot showing cleaved caspase 3. B. Immunoblot showing both full length and cleaved Parp-1. C. Immunoblot showing GAPDH. D. Immunoblot showing β-actin. (PPTX 110 kb) |
