Protective effect of bacterial lipase on lipopolysaccharide-induced toxicity in rat cardiomyocytes; H9C2 cell line
| Autor: | Reza Rahbarghazi, Mohammad Reza Yousefi, Ayda Pouyafar, Yousef Faridvand, Alireza Dehnad, Majid Khaksar, Reza Zarezadeh, Mina Mamipour |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lipopolysaccharide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lipase Diseases of the circulatory (Cardiovascular) system Medicine MTT assay Viability assay cell cytotoxicity Lipase Cytotoxicity Receptor Clonogenic assay rat cardiomyocytes biology business.industry lipopolysaccharide toll-like receptor signaling Molecular biology 030104 developmental biology chemistry RC666-701 030220 oncology & carcinogenesis Toxicity biology.protein Original Article lipids (amino acids peptides and proteins) Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of Cardiovascular and Thoracic Research, Vol 12, Iss 1, Pp 35-42 (2020) Journal of Cardiovascular and Thoracic Research |
| ISSN: | 2008-6830 2008-5117 |
| Popis: | Introduction: Cardiovascular system is highly sensitive to LPS-induced oxidative damage. This study aimed to show the inhibitory effect of bacterial Lipase on LPS-induced cardiomyoblasts toxicity. Methods: Rat cardiomyoblasts H9C2 were classified into Control, LPS (cells received 0.1, 1 and 10 μg/mL LPS) and LPS+ Lipase groups. In LPS+Lipase group, different concentrations of lipopolysaccharide were pre-incubated with 5 mg/mL bacterial lipase at 37˚C overnight prior to cell treatment. After 72 hours, cell viability was assessed by MTT assay. The expression of key genes related to toll-like receptor signaling pathways was assessed by real-time PCR assay. Percentage of fatty acids was evaluated in each group using gas chromatography assay. The levels of NO was also measured using the Griess reaction. Results: Data showed H9C2 cells viability was decreased after exposure to LPS in a dose-dependent manner (P Conclusion: Reduction in lipopolysaccharide-induced cytotoxicity is possibly related to lipase activity and reduction of modified lipopolysaccharide with toll-like receptor. |
| Databáze: | OpenAIRE |
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