Formulation of an antimicrobial superabsorbent powder that gels in situ to produce reactive oxygen
| Autor: | Erik A. B. Hughes, Thomas J. Hall, Liam M. Grover, Sophie C. Cox, Inês A. Barroso, Ioannis Azoidis |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Swine Starch medicine.drug_class Sodium polyacrylate Antibiotics Bioengineering Microbial Sensitivity Tests 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Biomaterials chemistry.chemical_compound Freeze-drying Anti-Infective Agents medicine Animals Humans Food science chemistry.chemical_classification Reactive oxygen species biology 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Anti-Bacterial Agents 0104 chemical sciences Oxygen chemistry Mechanics of Materials Staphylococcus aureus Powders 0210 nano-technology Bacteria |
| Zdroj: | Materials Science and Engineering: C. 118:111479 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2020.111479 |
| Popis: | The enzymatic oxidation of glucose to produce reactive oxygen species (ROS) provides honey with antimicrobial efficacy. This mechanism offers an alternative to traditional antibiotics; however, topical use of honey is limited due to its adherent and highly viscous properties. This study aims to overcome these issues by engineering a powder-based system that eases delivery and offers in situ activation of ROS. Starch based drying agents were utilised to enable freeze drying of a medical honey, with methylated-β-cyclodextrin (MCD) enabling the highest active incorporation (70%) while still producing a free-flowing powder. Addition of a superabsorbent, sodium polyacrylate (≤40%) was shown to facilitate in situ gelation of the powder, with an absorption capacity of up to 120.7 ± 4.5 mL g−1. Promisingly efficacy of the optimised superabsorbent powder was demonstrated in vitro against several clinically relevant Gram-negative and Gram–positive bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa). Alongside this no adverse effects were observed against human dermal fibroblasts. Application of the superabsorbent powder in an ex-vivo porcine wound model revealed capability to form a protective hydrogel barrier in less than 1 min. Overall, this novel ROS producing superabsorbent powder has potential to tackle topical infections without using traditional antibiotics. |
| Databáze: | OpenAIRE |
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