Differential expression of centrosomal proteins at different stages of human glioma
| Autor: | Fang-Yi Lin, Chia-Hua Chou, Joon-Khim Loh, Sheng-Long Howng, Chung-Ching Chio, Ann-Shung Lieu, Chia-Hung Wu, Yi-Ren Hong |
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| Rok vydání: | 2009 |
| Předmět: |
Gene isoform
Adult Male Cancer Research Aurora B kinase Mitosis Cell Cycle Proteins Biology lcsh:RC254-282 Glioma Cell Line Tumor Genetics medicine Humans RNA Messenger Aged Neoplasm Staging Centrosome Brain Neoplasms Reverse Transcriptase Polymerase Chain Reaction Human brain Middle Aged medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology Immunohistochemistry Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Microscopy Fluorescence Case-Control Studies Centrin Microtubule Proteins Research Article |
| Zdroj: | BMC Cancer BMC Cancer, Vol 10, Iss 1, p 268 (2010) |
| ISSN: | 1471-2407 |
| Popis: | Background High-grade gliomas have poor prognosis, requiring aggressive treatment. The aim of this study is to explore mitotic and centrosomal dysregulation in gliomas, which may provide novel targets for treatment. Methods A case-control study was performed using 34 resected gliomas, which were separated into low- and high-grade groups. Normal human brain tissue was used as a control. Using immunohistochemical analysis, immunofluorescent microscopy, and RT-PCR, detection of centrins 1 and 2, γ-tubulin, hNinein, Aurora A, and Aurora B, expression was performed. Analysis of the GBM8401 glioma cell line was also undertaken to complement the in vivo studies. Results In high-grade gliomas, the cells had greater than two very brightly staining centrioles within large, atypical nuclei, and moderate-to-strong Aurora A staining. Comparing with normal human brain tissue, most of the mRNAs expression in gliomas for centrosomal structural proteins, including centrin 3, γ-tubulin, and hNinein isoforms 1, 2, 5 and 6, Aurora A and Aurora B were elevated. The significant different expression was observed between high- and low-grade glioma in both γ-tubulin and Aurora A mRNA s. In the high-grade glioma group, 78.6% of the samples had higher than normal expression of γ-tubulin mRNA, which was significantly higher than in the low-grade glioma group (18.2%, p < 0.05). Conclusions Markers for mitotic dysregulation, such as supernumerary centrosomes and altered expression of centrosome-related mRNA and proteins were more frequently detected in higher grade gliomas. Therefore, these results are clinically useful for glioma staging as well as the development of novel treatments strategies. |
| Databáze: | OpenAIRE |
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