Characterization and humanization of a monoclonal antibody that neutralizes human leukocyte interferon: a candidate therapeutic for IDDM and SLE
Autor: | Timothy A. Stewart, K. Jin Kim, Leonard G. Presta, Verna Gibbs, Jadine Lai, Anan Chuntharapai, Xiaojian Huang |
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Rok vydání: | 2001 |
Předmět: |
Models
Molecular medicine.drug_class Immunology Molecular Sequence Data Alpha interferon Alpha (ethology) Enzyme-Linked Immunosorbent Assay Biosensing Techniques Immunoglobulin light chain Monoclonal antibody Biochemistry Immunoglobulin G law.invention Mice law Interferon medicine Immunology and Allergy Animals Humans Lupus Erythematosus Systemic Amino Acid Sequence Molecular Biology biology Dose-Response Relationship Drug Antibodies Monoclonal Interferon-alpha Hematology Interferon-beta Virology Recombinant Proteins Protein Structure Tertiary Diabetes Mellitus Type 1 Recombinant DNA biology.protein Antibody medicine.drug HeLa Cells |
Zdroj: | Cytokine. 15(5) |
ISSN: | 1043-4666 |
Popis: | We have developed a panel of murine monoclonal antibodies that recognize human interferon alpha. One of these mononclonal antibodies binds and neutralizes, with high affinity, all of seven tested recombinant human interferon alphas. This mononclonal antibody also neutralizes the interferon activity present in two independent pools of interferon alphas prepared following stimulation of human peripheral blood leukocytes. The complementary determining regions from this murine mononclonal antibody were transferred to a human IgG2 heavy chain and to a human kappa1 light chain. In addition, six (heavy chain) and two (light chain) amino acids were transferred from the framework regions. This generated a humanized mononclonal antibody that retained the specificity of the mouse parent. The humanized anti-interferon alpha antibody is a candidate therapeutic for those diseases, such as insulin-dependent diabetes, systemic lupus erythematosis, psoriasis and Crohn's disease, which are all characterized by pathological expression of interferon alpha. |
Databáze: | OpenAIRE |
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