Antisense-Guided Isolation and Structure Elucidation of Pannomycin, a Substituted cis-Decalin from Geomyces pannorum
| Autor: | Jenny M. Baxter, Maria Teresa Diez, Fernando Pelaez, Craig A. Parish, Kenneth E. Wilson, Samantha Tucker-Samaras, Scott K. Smith, Mercedes de la Cruz, Javier Collado, Deborah L. Zink, Gonzalo Platas, Francisca Vicente, Gerald F. Bills |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Staphylococcus aureus
Pharmaceutical Science Microbial Sensitivity Tests Naphthalenes Secondary metabolite Biology medicine.disease_cause Analytical Chemistry chemistry.chemical_compound Ascomycota Bacterial Proteins Decalin Drug Discovery medicine RNA Antisense Geomyces pannorum Adenosine Triphosphatases Pharmacology SecA Proteins Molecular Structure Strain (chemistry) Organic Chemistry Membrane Transport Proteins Stereoisomerism Isolation (microbiology) Anti-Bacterial Agents Antisense RNA medicine.drug_formulation_ingredient Secretory protein Complementary and alternative medicine chemistry Biochemistry Molecular Medicine SEC Translocation Channels medicine.drug |
| Zdroj: | Journal of Natural Products. 72:59-62 |
| ISSN: | 1520-6025 0163-3864 |
| Popis: | Antisense-based screening strategies can be used to sensitize a microorganism and selectively detect inhibitors against a particular cellular target of interest. A strain of Staphylococcus aureus that generates an antisense RNA against SecA, a central member of the protein secretion machinery, has been used to screen for novel antibacterials. Possible inhibitors of the SecA ATP-ase were selected with a high-throughput, two-plate agar-based whole cell differential sensitivity screen. After screening a library of over 115 000 natural products extracts with the SecA antisense strain, an extract of Geomyces pannorum was identified as providing increased activity against the sensitized strain as compared with the wild-type control. Bioassay-guided isolation of the active component from this fungal extract provided a new cis-decalin secondary metabolite, which we have named pannomycin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|