Subcutaneous Growth of Human Acoustic Schwannomas in Athymic Nude Mice
| Autor: | Mirko Tos, Jørgen Rygaard, Leif Klinken, Jens Thomsen, Samih Charabi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Pathology medicine.medical_specialty Transplantation Heterologous Mice Nude Mitosis Soft Tissue Neoplasms Cerebellopontine Angle Neovascularization Mice Nude mouse Antigen In vivo Proliferating Cell Nuclear Antigen medicine Animals Humans Cerebellar Neoplasms Neovascularization Pathologic Staining and Labeling biology business.industry S100 Proteins Nuclear Proteins Neuroma Acoustic General Medicine biology.organism_classification Neuroma medicine.disease Immunohistochemistry Neoplasm Proteins Proliferating cell nuclear antigen Transplantation Ki-67 Antigen Otorhinolaryngology Ki-67 biology.protein Female Schwann Cells medicine.symptom business Cell Division Neoplasm Transplantation |
| Zdroj: | Acta Oto-Laryngologica. 114:399-405 |
| ISSN: | 1651-2251 0001-6489 |
| Popis: | In order to develop an in vivo model for growth of acoustic schwannomas, we studied tumor specimens from 10 patients, transplanted into a subcutaneous pocket of 67 nude mice. The number of tumors which survived or grew was 63 (94%). Obvious macroscopic growth was observed in 22 (33%), status quo in 28 (42%), and regression of tumor size in 13 (19%). The tumor disappeared in 4 cases (6%). Serial implantation was not possible due to the small amount of neuroma tissue in the surviving tumors. In animals with obvious macroscopic growth, neovascularization was clearly demonstrated. The presence of Schwann cells in the implants was confirmed immunohistochemically. The proliferative activity in the original and implanted tumors was evaluated by the proliferating cell nuclear antigen (PCNA) and Ki-67 nuclear antigen stainings and showed good correlation between primary tumors and implants. This in vivo tumor model will open new opportunities to study the biology of acoustic tumors and to test different therapeutic modalities. |
| Databáze: | OpenAIRE |
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