Distribution of Vibrio cholerae virulence genes among different Vibrio species isolated in Sardinia, Italy
| Autor: | Leonardo Antonio Sechi, Stefania Anna Lucia Zanetti, Antonella Deriu, Ilaria Duprè, Guido Fadda |
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| Rok vydání: | 2000 |
| Předmět: |
Operon
Virulence medicine.disease_cause Applied Microbiology and Biotechnology Polymerase Chain Reaction Microbiology Vibrionaceae medicine Vibrio cholerae Phylogeny Vibrio Vibrio alginolyticus biology Vibrio parahaemolyticus General Medicine biology.organism_classification medicine.disease Cholera DNA Fingerprinting Italy Genes Bacterial Water Microbiology Biotechnology |
| Zdroj: | Journal of applied microbiology. 88(3) |
| ISSN: | 1364-5072 |
| Popis: | The members of the genus Vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. Diarrhoea caused by Vibrio cholerae is attributed to cholerae enterotoxin (CT) codified by the ctx operon and regulated by a number of virulence genes such as toxT, toxR and toxS. Fifty-two Vibrio strains were isolated from different aquatic environments in and around Sardinia and searched by PCR for the presence of ctxA, zot, ace, toxR, toxS, toxT, tcpA and vpi virulence genes in the genomes of the isolates. The toxR operon was found in 27 Vibrio alginolyticus strains out of 42 analysed, in three out of four V. cholerae non-O1 strains and in three Vibrio parahaemolyticus isolates. A positive amplification for the virulence pathogenic island (vpi) was produced by five V. alginolyticus strains. Finally, the ace expected amplification fragment was found in two V. alginolyticus isolates whereas the amplification with zot primers produced the expected fragment in one V. alginolyticus isolate. Differentiation of these strains with a PCR fingerprinting technique revealed no association between the presence of virulence genes and a particular fingerprinting pattern. Although most Vibrio species are considered non-pathogenic or only potentially harmful to humans, the finding of V. cholerae virulence genes in other members of the genus Vibrio, and the recent reports of the creation and evolution of pandemic strains of V. cholerae, may give a new perspective to the significance of these results. |
| Databáze: | OpenAIRE |
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