Relationship Between Gelatinases and Bone Turnover in the Healing Bone Defect
| Autor: | Hsi Ming Lee, Lorne M. Golub, Richard A. Reinhardt, Marian J. Schmid, Jeffrey B. Payne |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Oral Surgical Procedures Osteocalcin Alveolar Bone Loss Urology Collagen Type I Statistics Nonparametric Bone remodeling chemistry.chemical_compound N-terminal telopeptide Bone Density medicine Humans Therapeutic Irrigation Dental alveolus Inflammation Wound Healing Pyridinoline biology business.industry Soft tissue Middle Aged Gingivitis Otorhinolaryngology Trephine chemistry Gelatinases biology.protein Electrophoresis Polyacrylamide Gel Female Surgery Bone Remodeling Collagen Oral Surgery Peptides Wound healing business Biomarkers |
| Zdroj: | Journal of Oral and Maxillofacial Surgery. 63:1455-1460 |
| ISSN: | 0278-2391 |
| DOI: | 10.1016/j.joms.2005.05.319 |
| Popis: | The aim of this pilot study was to determine the relationship between gelatinase (MMP-9 and MMP-2) markers of soft tissue inflammation/turnover at the bone/soft tissue interface and bone turnover (osteocalcin [OC], pyridinoline cross-linked carboxyl-terminal telopeptide of type 1 collagen [ICTP], and bone fill) during healing of an alveolar bone defect.Ten subjects undergoing oral surgery had a 5 x 5-mm trephine defect created on an edentulous ridge and were sampled at the bone/soft tissue interface at baseline (prior to flap reflection), 2 weeks and 12 weeks postsurgery, using a novel bone wash device. Recovered irrigants were analyzed for MMP-9 and MMP-2 by gelatin zymography, OC and ICTP with radioimmunoassays, and albumin (ALB; to normalize markers for blood content) with a sandwich enzyme-linked immunosorbent assay. Bone fill at 12 weeks was analyzed by radiographic absorptiometry.All markers of enzymatic activity and bone turnover varied significantly across time (Por = .03), with bone turnover markers OC and ICTP decreasing between baseline and 2 weeks, and MMP-9 and MMP-2 increased. Measures generally returned to near baseline levels after 12 weeks. MMP-9 versus MMP-2 (r = 0.97, P.0001) and OC versus ICTP (r = 0.38, P = .048) were correlated with each other, while MMP-9 and MMP-2 were negatively correlated with ICTP (r = -0.48, P = .011 and r = -0.62, P = .006, respectively). MMP-9 was negatively correlated with subsequent bone fill (r = -0.63, P = .07).Bone wash sampling showed that gelatinase activity at 2 weeks following creation of an alveolar defect appeared to decrease bone turnover and eventual bone fill, suggesting benefits for anti-MMP therapy during wound healing. |
| Databáze: | OpenAIRE |
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