PCP degradation is mediated by closely related strains of the genus Sphingomonas
| Autor: | R. L. Crawford, R. P. Herwig, C. S. Orser, M. M. Ederer |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pentachlorophenol
Molecular Sequence Data DNA Ribosomal Flavobacterium Mixed Function Oxygenases Microbiology Phylogenetics Pseudomonas Sequence Homology Nucleic Acid Arthrobacter Genetics Amino Acid Sequence Conserved Sequence Phylogeny Ecology Evolution Behavior and Systematics Sphingolipids Base Sequence Gram-Negative Aerobic Bacteria biology Fatty Acids Nucleic acid sequence Sphingomonas biology.organism_classification 16S ribosomal RNA Blotting Southern Genes Bacterial Bacteria |
| Zdroj: | Molecular Ecology. 6:39-49 |
| ISSN: | 1365-294X 0962-1083 |
| DOI: | 10.1046/j.1365-294x.1997.00151.x |
| Popis: | There have been numerous reports in the literature of diverse bacteria capable of degrading pentachlorophenol (PCP). In order to gain further insight into the phylogenetic relationships of PCP-degrading bacteria, we examined four strains: Arthrobacter sp. strain ATCC 33790, Flavobacterium sp. strain ATCC 39723, Pseudomonas sp. strain SR3, and Sphingomonas sp. strain RA2. These organisms were isolated from different geographical locations and all of them degrade high concentrations (100-200 mg/L) of PCP. Southern blot analyses determined that these bacteria all harbour DNA that encodes similar, if not identical, genes involved in PCP degradation. Comparison of the 16S rRNA nucleotide sequences revealed that these organisms were very closely related and, in fact, represent a monophyletic group. The 16S rRNA analyses together with fatty acid and sphingolipid analyses strongly suggest that the four strains are members of the genus Sphingomonas. The close relationship of the four organisms is supported by nucleotide sequence analysis data of the pcpB locus encoding PCP-4-monooxygenase, the first enzyme in the PCP degradative pathway. |
| Databáze: | OpenAIRE |
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