Th1, Th2, Th17 and regulatory T cell pattern in psoriatic patients: modulation of cytokines and gene targets induced by etanercept treatment and correlation with clinical response
| Autor: | Renata Ponti, Maria Elena Terlizzi, Sara Astegiano, Rossana Cavallo, Maria Grazia Bernengo, Stefano Cicchelli, Mauro Novelli, E Barberio, Massimiliano Bergallo, Pietro Quaglino, E Buffa, Alessandra Comessatti, Cinzia Costa |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Regulatory T cell Dermatology Biology T-Lymphocytes Regulatory Receptors Tumor Necrosis Factor Etanercept Th2 Cells Psoriasis Gene expression medicine Humans Immunologic Factors RNA Messenger Receptor Transcription factor Cytokines Treg cells Gene targets Gene Expression Profiling Case-control study Middle Aged Th1 Cells medicine.disease Gene expression profiling medicine.anatomical_structure Treatment Outcome Case-Control Studies Immunoglobulin G Immunology Cancer research Th17 Cells Female medicine.drug Transcription Factors |
| Zdroj: | Dermatology (Basel, Switzerland). 223(1) |
| ISSN: | 1421-9832 |
| Popis: | Background: Psoriasis is sustained by pro-inflammatory CD4+ T helper cells mainly belonging to the Th1, Th17 and Th22 lineage. Objective: To identify whether treatment with the anti-tumour-necrosis-factor antagonist etanercept is able to induce significant modulations in transcription factor and cytokine mRNA gene expressions related to the different T cell immune response polarization (Th1, Th2, Th17 and regulatory T cells, Treg) and to correlate them with clinical response. Methods: The study population included 19 psoriasis patients treated with etanercept and 19 healthy subjects. Blood samples were collected at baseline and every 4 weeks during treatment. Taqman quantitative real-time polymerase chain reaction was applied to analyse the expression of: Stat-4, T-bet, IL-12p35 and IFN-γ (Th1-related); GATA-3, IL-4 (Th2-related); Stat-3, RORγt, IL-23p19 (Th17-related); Foxp3, IL-2 (Treg-related). Flow cytometry was applied to analyse CD4+CD25+brightFoxp3+ cells in peripheral blood. Results: Upregulation of Th1 and Th17 and downregulation of Treg subsets was found at baseline. The response to etanercept could be associated with a significant reversal of the Th1/Th17 activation, and a concomitant upregulation of Th2 and Treg subsets. Conclusion: Our data may contribute to a better understanding of the mechanisms underlying the achievement of clinical response in psoriasis and could be helpful for the identification of early predictive markers of response. |
| Databáze: | OpenAIRE |
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