WDR47 protects neuronal microtubule minus ends from katanin-mediated severing
| Autor: | Buijs, Robin R., Hummel, Jessica J.A., Burute, Mithila, Pan, Xingxiu, Cao, Yujie, Stucchi, Riccardo, Altelaar, Maarten, Akhmanova, Anna, Kapitein, Lukas C., Hoogenraad, Casper C., Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Celbiologie, Biomolecular Mass Spectrometry and Proteomics |
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| Přispěvatelé: | Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Celbiologie, Biomolecular Mass Spectrometry and Proteomics |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Dendrite Katanin Microtubules Biochemistry General Biochemistry Genetics and Molecular Biology dendrite Microtubule Chlorocebus aethiops medicine Animals Humans CAMSAP Rats Wistar Axon Biology (General) Cytoskeleton Neurons axon biology Chemistry Biochemistry Genetics and Molecular Biology(all) katanin WDR47 cytoskeleton Dendrites Axons Neuroprotection neuron Cell biology Microtubule minus-end HEK293 Cells medicine.anatomical_structure centrosome nervous system Centrosome Gene Knockdown Techniques COS Cells biology.protein Female Neuron Microtubule-Associated Proteins Protein Binding Genetics and Molecular Biology(all) microtubule |
| Zdroj: | Cell Reports, Vol 36, Iss 2, Pp 109371-(2021) Cell Reports, 36(2), 1. Elsevier Saunders |
| ISSN: | 2211-1247 |
| Popis: | Summary: Axons and dendrites are long extensions of neurons that contain arrays of noncentrosomal microtubules. Calmodulin-regulated spectrin-associated proteins (CAMSAPs) bind to and stabilize free microtubule minus ends and are critical for proper neuronal development and function. Previous studies have shown that the microtubule-severing ATPase katanin interacts with CAMSAPs and limits the length of CAMSAP-decorated microtubule stretches. However, how CAMSAP and microtubule minus end dynamics are regulated in neurons is poorly understood. Here, we show that the neuron-enriched protein WDR47 interacts with CAMSAPs and is critical for axon and dendrite development. We find that WDR47 accumulates at CAMSAP2-decorated microtubules, is essential for maintaining CAMSAP2 stretches, and protects minus ends from katanin-mediated severing. We propose a model where WDR47 protects CAMSAP2 at microtubule minus ends from katanin activity to ensure proper stabilization of the neuronal microtubule network. |
| Databáze: | OpenAIRE |
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