European Leucoma salicis NPV is closely related to North American Orgyia pseudotsugata MNPV
| Autor: | Monique M. van Oers, Jenny S. Cory, Just M. Vlak, Jadwiga Ziemnicka, Agata K. Jakubowska |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
anagrapha-falcifera
Baculoviridae Genes Viral Sequence analysis genome sequence Molecular Sequence Data Laboratory of Virology Polymerase Chain Reaction Laboratorium voor Virologie Lepidoptera genitalia baculovirus Sequence Homology Nucleic Acid sequence-analysis Botany Polyhedrin Animals Pest Control Biological gene Phylogeny Ecology Evolution Behavior and Systematics Genetics Base Sequence biology Leucoma salicis rachiplusia-ou fungi Nuclear Polyhedrosis Virus Sequence Analysis DNA PE&RC biology.organism_classification Nucleopolyhedroviruses spodoptera-exigua Lepidoptera nuclear polyhedrosis-virus identification Restriction fragment length polymorphism Orgyia pseudotsugata Polymorphism Restriction Fragment Length nucleopolyhedrovirus |
| Zdroj: | Journal of Invertebrate Pathology 88 (2005) 2 Journal of Invertebrate Pathology, 88(2), 100-107 |
| ISSN: | 0022-2011 |
| DOI: | 10.1016/j.jip.2004.12.002 |
| Popis: | The satin moth Leucoma salicis L. (Lepidoptera, Lymantriidae) is a frequent defoliator of poplar trees (Populus spp.) in Europe and Asia (China, Japan). Around 1920 the insect was introduced into the USA and Canada. In this paper, a multicapsid nucleopolyhedrovirus isolated from L. salicis larvae in Poland (LesaNPV) was characterized and appeared to be a variant of Orgyia pseudotsugata (Op) MNPV. O. pseudotsugata, the Douglas fir tussock moth (Lepidoptera, Lymantriidae), occurs exclusively in North America. Sequences of three conserved baculovirus genes, polyhedrin, lef-8, and pif-2, were amplified in polymerase chain reactions using degenerate primer sets, and revealed a high degree of homology to OpMNPV. Restriction enzyme analysis confirmed the close relationship between LesaNPV and OpMNPV, although a number of restriction fragment length polymorphisms were observed. The lef-7 gene, encoding late expression factor 7, and the ctl-2 gene, encoding a conotoxin-like protein, were chosen as putative molecular determinants of the respective viruses. The ctl-2 region appeared suitable for unequivocal identification of either virus as LesaNPV lacked a dUTPase gene in this region. Our observations may suggest that LesaNPV, along with L. salicis, was introduced into O. pseudotsugata after introduction of the former insect into North America in the 1920s. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect