Increased Expression of Retinol-Binding Protein 4 in Ovarian Endometrioma and Its Possible Role in the Pathogenesis of Endometriosis

Autor: Ju Hee Kim, Sa Ra Lee, Sung-Hoon Kim, Jae Chul Lee, Hee Dong Chae, Young Sang Oh
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
endometriosis
medicine.medical_treatment
Endometriosis
Uterus
Gene Expression
Pathogenesis
0302 clinical medicine
ovarian endometrioma
Medicine
Biology (General)
RNA
Small Interfering
Spectroscopy
030219 obstetrics & reproductive medicine
biology
General Medicine
Transfection
Immunohistochemistry
Recombinant Proteins
Computer Science Applications
Chemistry
medicine.anatomical_structure
Cytokine
Matrix Metalloproteinase 9
Matrix Metalloproteinase 2
Female
RNA Interference
Disease Susceptibility
Stromal cell
QH301-705.5
Cell Survival
Catalysis
Article
retinol-binding protein 4
Immunophenotyping
Inorganic Chemistry
03 medical and health sciences
Humans
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Retinol binding protein 4
adipokine
business.industry
Cell growth
Organic Chemistry
Ovary
medicine.disease
030104 developmental biology
Gene Expression Regulation
biology.protein
Cancer research
business
Retinol-Binding Proteins
Plasma
Biomarkers
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 5827, p 5827 (2021)
Volume 22
Issue 11
ISSN: 1422-0067
Popis: Although endometriosis is a benign disease characterized by the presence of endometrial tissues outside the uterus, ectopic endometrial cells can exhibit malignant biological behaviors. Retinol-binding protein4 (RBP4) is a novel adipocyte-derived cytokine, which has important roles in regulating insulin sensitivity and energy metabolism. RBP4 is a potent modulator of gene transcription, and acts by directly controlling cell growth, invasiveness, proliferation and differentiation. Here, we evaluated the possible role of RBP4 in the pathogenesis of endometriosis. We compared the levels of RBP4 in the tissues and peritoneal fluid (PF) of women with and without endometriosis and evaluated the in vitro effects of RBP4 on the viability, invasiveness, and proliferation of endometrial stromal cells (ESCs). RBP4 levels were significantly higher in the PF of the women in the endometriosis group than in the controls. RBP4 immunoreactivity was significantly higher in the ovarian endometriomas of women with advanced stage endometriosis than those of controls. In vitro treatment with human recombinant-RBP4 significantly increased the viability, bromodeoxyuridine expression, and invasiveness of ESCs. Transfection with RBP4 siRNA significantly reduced ESC viability and invasiveness. These findings suggest that RBP4 partakes in the pathogenesis of endometriosis by increasing the viability, proliferation and invasion of endometrial cells.
Databáze: OpenAIRE
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