Danger-recognizing proteins, β-defensin-128 and histatin-3, as potential biomarkers of recurrent coronary events
| Autor: | Rajani Kanth Vangala, Vijay V. Kakkar, Sangeeta Maity, Ankit Sharma, Madankumar Ghatge |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Proteomics 0301 basic medicine Oncology medicine.medical_specialty beta-Defensins Coronary Artery Disease Histatins 030204 cardiovascular system & hematology Bioinformatics 03 medical and health sciences 0302 clinical medicine Recurrence Internal medicine Genetics medicine Humans Prospective Studies Defensin Histatin 3 Proportional Hazards Models Framingham Risk Score business.industry Hazard ratio Cancer General Medicine Odds ratio Middle Aged medicine.disease 030104 developmental biology Proteome Female business Risk assessment Biomarkers |
| Zdroj: | International Journal of Molecular Medicine. 40:531-538 |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2017.3031 |
| Popis: | Conventional risk factors have limited ability to predict recurrent events in subjects with first-time coronary artery disease (CAD). This aim of this study was to identify novel biomarkers using comparative global proteome analysis to improve the risk assessment for recurrent coronary events. We used samples from phase-I of the Indian Atherosclerosis Research Study (IARS), consisting of 2,332 subjects, of whom 772 were CAD-affected subjects, including 152 with recurrent events identified during a 5-year follow-up period. Global proteome analysis was performed on serum samples of 85 subjects with recurrent coronary events and 85 age- and gender-matched subjects with first-time CAD using surface-enhanced laser desorption ionization time-of-flight mass spectrometry with CM10 arrays. TagIdent was used for protein identification followed by validation by western blot analysis and ELISA. Data were analyzed by logistic analysis, Cox-regression, hazards ratio, C-statistics and combined-marker risk score using SPSS version-17 and R-package version-2.13.0 software. We identified 16 significantly differentially expressed protein peaks. Of these, 2 peaks corresponding to m/z 8588 and 1864 were identified as β-defensin-128 and histatin-3, belonging to the danger-recognizing peptide family, which exhibited a significant independent association with recurrent events (odds ratios of 7.49 and 1.4, respectively). C-statistics improved significantly from 0.677 for conventional risk factors alone to 0.800 (p-value=0.001) in combination with β-defensin-128 and histatin-3 with a hazards ratio of 1.833. A combined risk score of β-defensin-128 and histatin-3 could reclassify 112 out of the 170 subjects into intermediate- and high-risk groups. On the whole, our data indicate that β-defensin-128 and histatin-3 may be potential biomarkers whch may be used to improve risk the stratification for recurrent coronary events. |
| Databáze: | OpenAIRE |
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