A rapid and sensitive method for determination of sorafenib in human plasma using a liquid chromatography/tandem mass spectrometry assay
| Autor: | Martin Radtke, Ping He, Sharyn D. Baker, Ming Zhao, Manuel Hidalgo, John Wright, Frank Thorsten Hafner, Wells A. Messersmith, B. Douglas Smith, Michelle A. Rudek |
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| Rok vydání: | 2007 |
| Předmět: |
Niacinamide
Spectrometry Mass Electrospray Ionization Electrospray Pyridines Formic acid Clinical Biochemistry Analytical chemistry Mass spectrometry Tandem mass spectrometry Sensitivity and Specificity Biochemistry Analytical Chemistry chemistry.chemical_compound Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Protein precipitation Sample preparation Protein Kinase Inhibitors Chromatography Phenylurea Compounds Benzenesulfonates Reproducibility of Results Cell Biology General Medicine Reference Standards Sorafenib chemistry Area Under Curve Calibration Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B. 846:1-7 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2006.06.005 |
| Popis: | A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sorafenib in human plasma. Sample preparation involved a single protein precipitation step by the addition of 0.1 mL of plasma with 0.5 mL acetonitrile. Analysis of the compounds of interest including the internal standard ([(2)H(3)(15)N] sorafenib) was achieved on a Waters X-Terra C(18) (150 mm x 2.1mm i.d., 3.5 microm) analytical column using a mobile phase consisting of acetonitrile/10 mM ammonium acetate (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.2 mL/min for 6 min. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 7.3-7260 ng/mL for the human plasma samples with values for the coefficient of determination of >0.96. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods ( |
| Databáze: | OpenAIRE |
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