Ubiquitin Ligase ATL31 Functions in Leaf Senescence in Response to the Balance Between Atmospheric CO2 and Nitrogen Availability in Arabidopsis
| Autor: | Yoshie Morita, Thais Huarancca Reyes, Junji Yamaguchi, Shoki Aoyama, Takeo Sato, Yu Lu, Lorenzo Guglielminetti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Senescence
Chlorophyll C/N balance Time Factors Physiology Nitrogen Ubiquitin-Protein Ligases Mutant Arabidopsis Down-Regulation Plant Science Anthocyanins chemistry.chemical_compound Gene Knockout Techniques Ubiquitin Gene Expression Regulation Plant Genes Reporter Gene expression Biomass Special Focus Issue – Regular Papers Photosynthesis Chlorosis biology Arabidopsis Proteins fungi food and beverages Starch Cell Biology General Medicine Carbon Dioxide biology.organism_classification Plants Genetically Modified Ubiquitin ligase Up-Regulation DNA-Binding Proteins Plant Leaves Phenotype chemistry Biochemistry Mutation biology.protein CO2 |
| Zdroj: | Plant and Cell Physiology |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | Carbon (C) and nitrogen (N) are essential elements for metabolism, and their availability, called the C/N balance, must be tightly coordinated for optimal growth in plants. Previously, we have identified the ubiquitin ligase CNI1/ATL31 as a novel C/N regulator by screening plants grown on C/N stress medium containing excess sugar and limited N. To elucidate further the effect of C/N balance on plant growth and to determine the physiological function of ATL31, we performed C/N response analysis using an atmospheric CO2 manipulation system. Under conditions of elevated CO2 and sufficient N, plant biomass and total sugar and starch dramatically increased. In contrast, elevated CO2 with limited N did not increase plant biomass but promoted leaf chlorosis, with anthocyanin accumulation and increased senescence-associated gene expression. Similar results were obtained with plants grown in medium containing excess sugar and limited N, suggesting that disruption of the C/N balance affects senescence progression. In ATL31-overexpressing plants, promotion of senescence under disrupted CO2/N conditions was repressed, whereas in the loss-of-function mutant it was enhanced. The ATL31 gene was transcriptionally up-regulated under N deficiency and in senescent leaves, and ATL31 expression was highly correlated with WRKY53 expression, a key regulator of senescence. Furthermore, transient protoplast analysis implicated the direct activation of ATL31 expression by WRKY53, which was in accordance with the results of WRKY53 overexpression experiments. Together, these results demonstrate the importance of C/N balance in leaf senescence and the involvement of ubiquitin ligase ATL31 in the process of senescence in Arabidopsis. |
| Databáze: | OpenAIRE |
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