Digital PCR improves the quantitation of DMR and the selection of CML candidates to TKIs discontinuation

Autor:	Mario Tiribelli, Chiara Cattaneo, Massimiliano Bonifacio, Fabio Stagno, Cristina Bucelli, Luigi Scaffidi, Gianluca Gaidano, Mariella D'Adda, Mirko Farina, Domenico Russo, Eleonora Toffoletti, Elif Dereli Eke, Clara Deambrogi, Simona Bernardi, Francesco Di Raimondo, Michele Malagola, Marco Gobbi, Alessandra Iurlo, Luca Franceschini, Maria Domenica Divona, Nicola Polverelli, Camilla Zanaglio, Micaela Bergamaschi, Elisabetta Abruzzese
Rok vydání:	2019
Předmět:	Male 0301 basic medicine Cancer Research Neoplasm Residual Fusion Proteins bcr-abl Polymerase Chain Reaction Gastroenterology Cohort Studies 0302 clinical medicine hemic and lymphatic diseases Digital polymerase chain reaction Original Research Therapeutic strategy Aged 80 and over Myeloid leukemia Middle Aged lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Gene Expression Regulation Neoplastic Treatment Outcome Real-time polymerase chain reaction digital PCR (dPCR) Oncology 030220 oncology & carcinogenesis Cohort Female chronic myeloid leukemia minimal residual disease (MRD) monitoring treatment-free remission (TFR) tyrosine kinase inhibitors (TKI) discontinuation treatment‐free remission (TFR) Adult medicine.medical_specialty lcsh:RC254-282 Sensitivity and Specificity Young Adult 03 medical and health sciences Leukemia Myelogenous Chronic BCR-ABL Positive Internal medicine medicine Humans Radiology Nuclear Medicine and imaging In patient Protein Kinase Inhibitors Aged business.industry Significant difference Clinical Cancer Research Discontinuation 030104 developmental biology business
Zdroj:	Cancer Medicine Cancer Medicine, Vol 8, Iss 5, Pp 2041-2055 (2019)
ISSN:	2045-7634
DOI:	10.1002/cam4.2087
Popis:	Treatment‐free remission (TFR) by tyrosine kinase inhibitors (TKI) discontinuation in patients with deep molecular response (DMR) is a paramount goal in the current chronic myeloid leukemia (CML) therapeutic strategy. The best DMR level by real‐time quantitative PCR (RT‐qPCR) for TKI discontinuation is still a matter of debate. To compare the accuracy of digital PCR (dPCR) and RT‐qPCR for BCR‐ABL1 transcript levels detection, 142 CML patients were monitored for a median time of 24 months. Digital PCR detected BCR‐ABL1 transcripts in the RT‐qPCR undetectable cases. The dPCR analysis of the samples, grouped by the MR classes, revealed a significant difference between MR4.0 and MR4.5 (P = 0.0104) or MR5.0 (P = 0.0032). The clinical and hematological characteristics of the patients grouped according to DMR classes (MR4.0 vs MR4.5‐5.0) were superimposable. Conversely, patients with dPCR values
Databáze:	OpenAIRE
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