Utilization of TALEN and CRISPR/Cas9 technologies for gene targeting and modification
| Autor: | David Frescas, Jiali Pu, Baorong Zhang, Jian Feng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetics
Nuclease Transcription activator-like effector nuclease biology Effector Genome Human Induced Pluripotent Stem Cells Gene targeting Minireviews Genome General Biochemistry Genetics and Molecular Biology Genome editing Gene Targeting biology.protein CRISPR Animals Humans Clustered Regularly Interspaced Short Palindromic Repeats Induced pluripotent stem cell |
| Popis: | The capability to modify the genome precisely and efficiently offers an extremely useful tool for biomedical research. Recent developments in genome editing technologies such as transcription activator-like effector nuclease and the clustered regularly interspaced short palindromic repeats system have made genome modification available for a number of organisms with relative ease. Here, we introduce these genome editing techniques, compare and contrast each technical approach and discuss their potential to study the underlying mechanisms of human disease using patient-derived induced pluripotent stem cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|