Norovirus Persistence in Oysters to Prolonged Commercial Purification
| Autor: | Elisabetta Suffredini, Érica Azevedo Costa, Laura Serracca, Valeria Listorti, Aitor Garcia-Vozmediano, Cristiana Maurella, Roberta Battistini, Mino Orlandi, Francesco Iacona, Carlo Ercolini, Chiara Masotti |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Veterinary medicine norovirus real-time qPCR Biology medicine.disease_cause Article Persistence (computer science) 03 medical and health sciences stomatognathic system medicine Immunology and Allergy Molecular Biology 030304 developmental biology 0303 health sciences General Immunology and Microbiology 030306 microbiology Crassostrea gigas Contamination depuration food safety Infectious Diseases Norovirus Medicine |
| Zdroj: | Pathogens Pathogens, Vol 10, Iss 944, p 944 (2021) Volume 10 Issue 8 |
| ISSN: | 2076-0817 |
| Popis: | Depuration is generally the main treatment employed for bivalve mollusks harvested from contaminated sites. Commercial depuration has demonstrated to be effective for removal of bacterial pathogens, although it probably provides only limited efficacy against human enteric viruses. We evaluated the quantitative reduction of norovirus (NoV) genogroups I and II in naturally contaminated oysters after 1, 4, and 9 days of depuration. The process was conducted in an authorized depuration plant, and NoV concentration was determined by RT-qPCR according to ISO 15216-1:2017 method. Regardless of the NoV genogroup, our results showed no significant reduction in NoV concentration after 1 day of depuration. Higher mean reduction (68%) was obtained after 4 days of treatment, while no further increase was observed after 9 days. Overall, reduction was highly variable, and none of the trials showed statistically significant reduction in NoV RNA concentration at the end of each depuration period. Indeed, NoV concentration remained high in 70% of samples even after 9 days of depuration, with values ranging between 4.0 × 102 and 2.3 × 104 g.c./g. These results indicate that an extension of commercial depuration time does not appear to be effective for reducing or eliminating NoV in oysters. |
| Databáze: | OpenAIRE |
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