Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
| Autor: | Veena Somasundaram, Timothy Back, Nimit L. Patel, Debashree Basudhar, Joseph D. Kalen, Robert Y.S. Cheng, David A. Wink, Lisa A. Ridnour |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lung Neoplasms General Chemical Engineering Antineoplastic Agents Triple Negative Breast Neoplasms Article General Biochemistry Genetics and Molecular Biology Metastasis Mice 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Line Tumor Animals Humans Medicine Survival rate Triple-negative breast cancer General Immunology and Microbiology business.industry General Neuroscience Cancer medicine.disease Xenograft Model Antitumor Assays Primary tumor Phenotype 030104 developmental biology Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer cell Cancer research Female business Ex vivo |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| Popis: | Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less aggressive breast tumors, the 5-year survival rate of TNBC patients is 77% due to their characteristic drug-resistant phenotype and metastatic burden. Toward this end, murine models have been established aimed at identifying novel therapeutic strategies limiting TNBC tumor growth and metastatic spread. This work describes a practical guide for the TNBC orthotopic model where MDA-MB-231 breast cancer cells suspended in a basement membrane matrix are implanted in the fourth mammary fat pad, which closely mimics the cancer cell behavior in humans. Measurement of tumors by caliper, lung metastasis assessment via in vivo and ex vivo imaging, and molecular detection are discussed. This model provides an excellent platform to study therapeutic efficacy and is especially suitable for the study of the interaction between the primary tumor and distal metastatic sites. |
| Databáze: | OpenAIRE |
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