M13 and pUC vectors with new unique restriction sites for cloning
| Autor: | Zdeněk Hostomský, Vladimir Benes, Václav Pačes, Luboš Arnold |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Genetic Vectors
Molecular Sequence Data Restriction Mapping DNA Recombinant Molecular cloning Bacteriophage Plasmid Genetics Amino Acid Sequence Vector (molecular biology) Cloning Molecular Deoxyribonucleases Type II Site-Specific Cloning Base Sequence biology Nucleic acid sequence DNA Restriction Enzymes General Medicine biology.organism_classification Mutagenesis Insertional Restriction enzyme Restriction site Oligodeoxyribonucleotides Mutagenesis Site-Directed Bacteriophage M13 Plasmids |
| Zdroj: | Gene. 130:151-152 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(93)90360-f |
| Popis: | Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessential part of the vector molecule was changed into a BssHII site, so that the NarI site in the MCS became unique. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|