Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b
| Autor: | Pertti Panula, Kirsi Sainio, Hannu Sariola, Tiina Immonen, Nina Perälä, Henri Koivula, Nina Peitsaro, Maria Sundvik |
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| Rok vydání: | 2010 |
| Předmět: |
Nervous system
Central Nervous System animal structures Central nervous system Molecular Sequence Data Embryonic Development Nerve Tissue Proteins 03 medical and health sciences 0302 clinical medicine Mesencephalon Cerebellum medicine Animals Amino Acid Sequence Zebrafish Phylogeny 030304 developmental biology Genetics 0303 health sciences Gene knockdown biology Behavior Animal Plexin Neural tube Gene Expression Regulation Developmental Exons Zebrafish Proteins biology.organism_classification Axons Introns Cell biology Rhombencephalon medicine.anatomical_structure nervous system embryonic structures biology.protein Axon guidance Neural development Cell Adhesion Molecules 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 239(10) |
| ISSN: | 1097-0177 |
| Popis: | In mice lacking Plexin B2, a receptor of the axon guidance molecules Semaphorin 4C and Semaphorin 4D, the closure of the neural tube and structural organization of the cerebellum are severely impaired. We cloned two Plexin B2 orthologs, plxnb2a and plxnb2b, in zebrafish, which is a widely used model for the development of the vertebrate central nervous system (CNS). The predicted proteins, Plexin B2a and Plexin B2b, contain all the conserved and functional domains of the plexin B-subfamily. During embryonic development, plxnb2a is expressed, e.g., in pharyngeal arches while plxnb2b expression is more confined to neuronal structures like the cerebellum. However, both plxnb2a and plxnb2b are expressed at the midbrain–hindbrain boundary, in the otic vesicles, facial ganglia, and pectoral fins. Knockdown of both plxnb2a and plxnb2b simultaneously (>95% and 45%, respectively) resulted in normal CNS structure, axon guidance and swimming performance of the morphants. Developmental Dynamics 239:2722–2734, 2010. © 2010 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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