Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori
| Autor: | Baolong Niu, Guoliang Fu, Li Jin, Qiuhong Guo, Zhiqi Meng, Anjiang Tan, Zhiqian Li, Luke Alphey, Neil I. Morrison, Yongping Huang |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Transgene Immunoblotting Silk Sexing Breeding Bombyx mori Animals Sericulture Cloning Molecular Pest Control Biological Gene DNA Primers Bombyx Genetics Cloning Sex Characteristics Larva Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction fungi Sequence Analysis DNA Biological Sciences Tetracycline biology.organism_classification Alternative Splicing Microscopy Fluorescence Trans-Activators Female Genes Lethal |
| Zdroj: | Proceedings of the National Academy of Sciences. 110:6766-6770 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori . We developed a construct in which a positive feedback loop regulated by sex-specific alternative splicing leads to high-level expression of the tetracycline-repressible transactivator in females only. Transgenic animals show female-specific lethality during embryonic and early larval stages, leading to male-only cocoons. This transgene-based female-specific lethal system not only has wide application in sericulture, but also has great potential in lepidopteran pest control. |
| Databáze: | OpenAIRE |
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