Arbovirus-derived piRNAs exhibit a ping-pong signature in mosquito cells
| Autor: | Pascal Miesen, Koen W. R. van Cleef, Maria-Carla Saleh, Nicolas Vodovar, Alfred W. Bronkhorst, Ronald P. van Rij, Hervé Blanc |
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| Přispěvatelé: | Virus et Interférence ARN - Viruses and RNA Interference, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Department of Microbiology and Immunology, Radboud University Medical Center [Nijmegen], This work was financially supported by the French Agence Nationale de la Recherche (ANR-09-394 JCJC-0045-01) and the European Research Council (FP7/2007-2013 ERC 242703) to M.C.S., and a VIDI fellowship from the Netherlands Organization for Scientific Research (project number 864.08.003) and Horizon Breakthrough fellowship from the Netherlands Genomics Initiative (project number 93519018) to RPvR., ANR-09-JCJC-0045,VIA(2009), European Project: 242703,EC:FP7:ERC,ERC-2009-StG,ANTIVIRALRNAI(2009), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2012 |
| Předmět: |
Small interfering RNA
MESH: Gene Amplification*/physiology MESH: DNA Transposable Elements/physiology viruses MESH: RNA Small Interfering/genetics MESH: Base Sequence Biochemistry MESH: Arboviruses/genetics arboviral infections Aedes RNA interference Nucleic Acids Molecular Cell Biology SiRNA MESH: Arbovirus Infections/virology MESH: Animals RNA Small Interfering MESH: Gene Amplification*/genetics Cells Cultured MESH: Culicidae/virology Genetics 0303 health sciences Multidisciplinary 3. Good health Antisense RNA Insects MESH: Culicidae/genetics MESH: Virus Integration/genetics [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine MESH: Arboviruses/physiology MESH: Aedes/cytology Research Article MESH: Cells Cultured Transposable element endocrine system Science Virus Integration Piwi-interacting RNA mosquito Aedes aegypti Arbovirus Infections Biology Microbiology Arbovirus MESH: Gene Expression Profiling 03 medical and health sciences medicine Animals 030304 developmental biology Base Sequence MESH: Aedes/virology 030306 microbiology urogenital system Gene Expression Profiling fungi Immunity Gene Amplification RNA Pathogenesis and modulation of inflammation Infection and autoimmunity [N4i 1] biology.organism_classification medicine.disease MESH: Aedes/genetics Culicidae MESH: RNA Small Interfering/metabolism MESH: Arbovirus Infections/genetics DNA Transposable Elements Arboviruses |
| Zdroj: | PLoS One, 7 PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (1), pp.e30861. ⟨10.1371/journal.pone.0030861.t001⟩ PLoS ONE, 2012, 7 (1), pp.e30861. ⟨10.1371/journal.pone.0030861.t001⟩ PLoS ONE, Vol 7, Iss 1, p e30861 (2012) PLoS One, 7, 1 |
| ISSN: | 1932-6203 |
| Popis: | Contains fulltext : 109736.pdf (Publisher’s version ) (Open Access) The siRNA pathway is an essential antiviral mechanism in insects. Whether other RNA interference pathways are involved in antiviral defense remains unclear. Here, we report in cells derived from the two main vectors for arboviruses, Aedes albopictus and Aedes aegypti, the production of viral small RNAs that exhibit the hallmarks of ping-pong derived piwi-associated RNAs (piRNAs) after infection with positive or negative sense RNA viruses. Furthermore, these cells produce endogenous piRNAs that mapped to transposable elements. Our results show that these mosquito cells can initiate de novo piRNA production and recapitulate the ping-pong dependent piRNA pathway upon viral infection. The mechanism of viral-piRNA production is discussed. |
| Databáze: | OpenAIRE |
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