Characterization of inhibin immunoreactivity in post-menopausal women with ovarian tumours
| Autor: | N. Cahir, P. Mamers, Tom Jobling, Henry G. Burger, David L. Healy, Nigel P. Groome, David Robertson |
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| Rok vydání: | 1996 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Granulosa cell Radioimmunoassay Heterologous Ovary Enzyme-Linked Immunosorbent Assay Peptide hormone Biology Cross Reactions Endocrinology Isomerism Internal medicine medicine Humans Inhibins Ovarian tumours Granulosa Cell Tumor Ovarian Neoplasms Middle Aged medicine.disease Adenocarcinoma Mucinous Cystadenocarcinoma Serous Postmenopause Serous fluid medicine.anatomical_structure Female Ovarian cancer |
| Zdroj: | Clinical endocrinology. 44(4) |
| ISSN: | 0300-0664 |
| Popis: | BACKGROUND AND OBJECTIVE We have previously reported elevated serum immunoreactive inhibin (INH) levels in patients with ovarian malignancies, particularly granulosa cell and mucinous tumours. The present study was designed to compare INH measurements using a heterologous radioimmunoassay with cross-reactivity for inhibin α-subunit derived peptides with measurements obtained using a new ELISA specific for dimeric inhibin-A. It was hypothesized that granulosa cell tumours may secrete significant quantities of inhibin-A whereas mucinous tumours were unlikely to do so because of the lack of a relation between INH and FSH measurements in the latter group. DESIGN Serum samples obtained from women found to have ovarian cancer were assayed using the heterologous radioimmunoassay (the Monash assay) and using an ELISA specific for dimeric inhibin (the Groome assay) and the results were compared. PATIENTS Samples for assay were available from 69 normal post-menopausal control women, 12 patients with mucinous tumours of the ovary, 26 with serous tumours, 7 with granulosa cell tumours and 8 with various other ovarian tumours. Patients were post-menopausal or had been subjected to bilateral oophorectomy at the time these samples were collected. MEASUREMENTS The Monash and Groome assays were carried out as described previously. The upper limit of normal for post-menopausal women in the Monash assay was 122 U/l and for the Groome assay was calculated to be 32 ng/l. RESULTS Among the 69 normal subjects, 4 were found to have elevated inhibin levels using the Monash RIA (133–190 U/l) and 4 were found to have elevated levels in the Groome ELISA (45.5–55.3 ng/l). Among 12 patients with mucinous tumours, 10 (83%) had elevated inhibin levels using the Monash assay but only 3 (25%) had elevated levels with the Groome assay (P |
| Databáze: | OpenAIRE |
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