Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion
| Autor: | Shinji Tanigaki, Hitoshi Ishimoto, Mamoru Tanaka, Yasunori Yoshimura, Toyohiko Miyazaki, Kei Miyakoshi, Michiya Natori, Osamu Nishimura |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Physiology Placenta Uterus Ischemia Biology Lesion Pregnancy Physiology (medical) Internal medicine Leukocytes medicine Animals Fetal Growth Retardation Antibodies Monoclonal Organ Size medicine.disease Lymphocyte Function-Associated Antigen-1 Rats Endocrinology medicine.anatomical_structure Fetal Weight In utero CD18 Antigens Reperfusion Injury Gestation Female medicine.symptom Cardiology and Cardiovascular Medicine Reperfusion injury Perfusion |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 280:H1215-H1221 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.2001.280.3.h1215 |
| Popis: | We investigated leukocyte involvement in uterine hypoperfusion and intrauterine fetal growth retardation (IUGR) induced by ischemia-reperfusion (I/R) in Sprague-Dawley rats. On day 17 of gestation, leukocyte accumulation in the uterus and placenta subjected to 30 min of ischemia, followed by reperfusion, was assessed by measuring myeloperoxidase (MPO) activity. Uterine MPO activity was significantly higher after 1 h of reperfusion than it was before ischemia ( P < 0.05), without any increase in placental MPO activity. Immunohistochemical staining showed leukocyte accumulation in the uterus subjected to I/R. The effects of treatment with monoclonal antibodies against CD11a (WT1) and CD18 (WT3) at a dose of 0.8 mg/kg on uterine blood flow and IUGR were investigated. Laser-Doppler flowmetry demonstrated that uterine hypoperfusion at 2 h after ischemia (blood flow, −51.7 ± 1.2%; P < 0.01) was inhibited by WT1 and WT3 treatment. I/R-induced IUGR at full term ( P < 0.05 vs. nonischemic horn) was prevented by WT1 and WT3 treatment on day 17. These results indicate that leukocyte accumulation may play an important role in the pathogenesis of uterine hypoperfusion and IUGR induced by I/R in pregnant rats. |
| Databáze: | OpenAIRE |
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