Labile iron pool and ferritin content in developing rat brain gamma-irradiated in utero
| Autor: | Susana Puntarulo, Elizabeth Robello, Andrea Galatro |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Iron
Biology Toxicology Nitric Oxide law.invention Nitric oxide Ciencias Biológicas chemistry.chemical_compound Western blot law Pregnancy medicine Animals Rats Wistar Electron paramagnetic resonance Fetus medicine.diagnostic_test General Neuroscience IRON Brain Bioquímica y Biología Molecular Molecular biology Fluorescence Rats Calcein Ferritin chemistry In utero Gamma radiation Gamma Rays Immunology Ferritins biology.protein Female FETAL RAT BRAIN FERRITIN NITRIC OXIDE CIENCIAS NATURALES Y EXACTAS Whole-Body Irradiation |
| Zdroj: | Neurotoxicology. 30(3) |
| ISSN: | 1872-9711 |
| Popis: | This study was aimed to assess the content of total Fe, Ferritin (Ft) and labile Fe pool (LIP) in developing rat brain exposed in utero to 1 Gy of gamma irradiation. A significant increase (2.3-fold) in the total Fe content of the fetal rat brain irradiated in utero was observed from 1 to 4 h post-irradiation, as compared to the content in non-irradiated brain. Ft was analyzed by immunoblotting. The Ft protein was composed by 20 kDa subunits. According to the analysis of the band density in the Western blot, the Ft content decreased by 77 ± 15% 2 h after gamma-irradiation, as compared to the values in non-irradiated samples. The effect of gamma-irradiation on the LIP was studied by both electron paramagnetic resonance (EPR) and by a fluorescence technique employing calcein (CA). A reduction on the LIP was detected at 2 h post-irradiation, independently of the methodology employed for the assay. Since NO content increased in the same time frame of LIP decreasing, a protective role for NO is suggested in fetal rat brain exposed to gamma-irradiation. The data presented in this work are the first experimental evidence suggesting that, as part of the network of the cellular response to limit irradiation-dependent injury, a complex interaction between Fe and NO could be triggered. Fil: Robello, Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Puntarulo, Susana Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina |
| Databáze: | OpenAIRE |
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