Group IB phospholipase A2 from Pseudonaja textilis
| Autor: | Siaw Ching Chai, Arunmozhiarasi Armugam, Phui Yee Siew, NanLing Gong, Ramkishen Nair, Kandiah Jeyaseelan, XiaoJie Li |
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| Rok vydání: | 2004 |
| Předmět: |
Gene isoform
Sequence analysis Thrombin Time Molecular Sequence Data Biophysics Venom Biochemistry Catalysis Mass Spectrometry Phospholipases A Pseudonaja textilis Exon Animals Amino Acid Sequence Elapidae Blood Coagulation Molecular Biology Gene Phylogeny Elapid Venoms Phospholipase A Base Sequence Sequence Homology Amino Acid biology Circular Dichroism Intron biology.organism_classification Molecular biology Isoenzymes Phospholipases A2 Protein Subunits Genes Prothrombin Time Sequence Alignment |
| Zdroj: | Archives of Biochemistry and Biophysics. 421:10-20 |
| ISSN: | 0003-9861 |
| Popis: | Pseudonaja textilis , an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A 2 in this venom. Besides being non-lethal, the phospholipase A 2 proteins were found to be moderately active enzymes and they showed procoagulant property. cDNA cloning and characterization indicated the presence of two isoforms of PLA 2 proteins in a single snake, each containing the “pancreatic loop,” characteristic of group IB phospholipase A 2 . The genomic cloning also confirmed the presence of two genes each containing four exons that are interrupted by three introns. Phylogenetic analysis showed that the venom group IB PLA 2 gene is primitive and could have evolved from the same ancestor as the mammalian and venom group IA PLA 2 genes. In the present study, we report that the Pt-PLA2 gene could be responsible for the production of PL1, 2, and 3 possibly via RNA editing process. |
| Databáze: | OpenAIRE |
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