Extrusion Printed Silk Fibroin Scaffolds with Post-mineralized Calcium Phosphate as a Bone Structural Material

Autor: Ruya Shi, Xingxing Cai, Guanping He, Juan Guan, Yuzeng Liu, Hongyi Lu, Zhinan Mao, Yan Li, Hongbo Guo, Yong Hai
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Bioprinting. 8
ISSN: 2424-8002
2424-7723
DOI: 10.18063/ijb.v8i4.596
Popis: Artificial bone materials are of high demand due to the frequent occurrence of bone damage from trauma, disease, and ageing. Three-dimensional (3D) printing can tailor-make structures and implants based on biomaterial inks, rendering personalized bone medicine possible. Herein, we extrusion-printed 3D silk fibroin (SF) scaffolds using mixed inks from SF and sodium alginate (SA), and post-mineralized various calcium phosphates to make hybrid SF scaffolds. The effects of printing conditions and mineralization conditions on the mechanical properties of SF scaffolds were investigated. The SF scaffolds from ~10 wt% SF ink exhibited a compressive modulus of 240 kPa, which was elevated to ~1600 kPa after mineralization, showing a significant reinforcement effect. Importantly, the mineralized SF 3D scaffolds exhibited excellent MC3T3-E1 cell viability and promoted osteogenesis. The work demonstrates a convenient strategy to fabricate SF-based hybrid 3D scaffolds with bone-mimetic components and desirable mechanical properties for bone tissue engineering.
Databáze: OpenAIRE
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