Feasibility of an automated coagulation factor XIIIa test using its isopeptidase activity
| Autor: | Martina Leitner, Christian Büchold, Nikolaus B. Binder, Ralf Pasternack |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Tissue transglutaminase
Biophysics Plasma Transglutaminase 01 natural sciences Biochemistry Coagulation factor XIIIa Automation 03 medical and health sciences Carbon-Nitrogen Lyases medicine Humans Acquired deficiency Molecular Biology 030304 developmental biology 0303 health sciences Plasma samples biology business.industry 010401 analytical chemistry Cell Biology Factor XIII medicine.disease 0104 chemical sciences Bleeding diathesis Isopeptidase activity Proteolysis biology.protein Feasibility Studies Factor XIIIa business medicine.drug Biomedical engineering |
| Zdroj: | Analytical Biochemistry. 600:113757 |
| ISSN: | 0003-2697 |
| Popis: | Plasma transglutaminase FXIII provides mechanical and biochemical stability to blood clots. Congenital or acquired deficiency may be associated with bleeding diathesis and requires therefore careful monitoring. The precise automated measurement of a large number of plasma samples can provide new insights regarding the clinical relevance of certain FXIII levels. There is still the unmet diagnostic need for a reliable high-throughput method. Here we report the development and feasibility study of a promising prototype, adapting the precise FXIIIa isopeptidase assay principle on the optimized automated Ceveron s100 platform. |
| Databáze: | OpenAIRE |
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