Development and validation of ultra‐high performance supercritical fluid chromatography method for quantitative determination of six compounds in Guizhi Fuling capsule and tablet samples
| Autor: | Lin Fan Tan, Ai-Jun Ma, Na Feng, Yuan Lin, Bao Qiong Li, Ze Ying Li, Xin Kang Li, Xiang-Zhi Zhang, Chao Yang Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Analyte
Chromatography Resolution (mass spectrometry) Propanols Amygdalin Acetophenones Capsules Chromatography Supercritical Fluid Filtration and Separation Paeoniflorin Cinnamic acid Analytical Chemistry chemistry.chemical_compound Glucosides chemistry Cinnamates Coumarins Monoterpenes Supercritical fluid chromatography Methanol Paeonol Drugs Chinese Herbal Tablets |
| Zdroj: | Journal of Separation Science. 44:3199-3207 |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.202100181 |
| Popis: | A fast and simple ultra-high performance supercritical fluid chromatography method has been developed for the determination of six analytes, namely (paeonol, coumarin, cinnamic alcohol, cinnamic acid, paeoniflorin and amygdalin) in Guizhi Fuling capsule and tablet samples. The influence of the key chromatographic parameters for the separation purposes was evaluated. The optimal column was Trefoil CEL1 column. The optimal mobile phase was a gradient mixture of carbon dioxide and methanol at flow rate of 1.0 mL min-1 . The back pressure of the system was set to 1.38×107 Pa and the temperature to 45 ℃. The six compounds were separated within 11 min by the proposed ultra-high performance supercritical fluid chromatography method with satisfactory resolution. Method validation confirmed that the procedure is accurate with the recovery rates from 87.04% to 104.30%, intra-day precision values less than less than 4.81% and inter-day precision less than 5.22%, and linear with R2 higher than 0.9967. Therefore, this work provides a simple and novel method for the simultaneous analysis of six compounds in Guizhi Fuling capsule and tablet samples. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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