Comparison of the metabolism of α-linolenic acid and its Δ6 desaturation product, stearidonic acid, in cultured NIH-3T3 cells
| Autor: | Gregory W. Ells, Yung-Sheng Huang, David F. Horrobin, R.C. Cantrill |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Linolenic Acids
Linolenic acid Eicosatetraenoic acid Arachidonic Acids Biology Biochemistry Mice chemistry.chemical_compound Fatty Acids Omega-3 Animals Phospholipids Triglycerides Dose-Response Relationship Drug alpha-Linolenic acid Fatty Acids Organic Chemistry alpha-Linolenic Acid 3T3 Cells Cell Biology Eicosapentaenoic acid Oleic acid Eicosapentaenoic Acid chemistry Docosahexaenoic acid Fatty Acids Unsaturated Docosapentaenoic acid Cell Division Stearidonic acid |
| Zdroj: | Lipids. 28:163-166 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/bf02536634 |
| Popis: | The incorporation and metabolism of alpha-linolenic acid (18:3n-3) and its delta 6 desaturase product, stearidonic acid (18:4n-3), were compared by NIH-3T3 cells. In the presence of fetal calf serum, cells accumulated exogenously added 18:3n-3 and 18:4n-3 apparently at the expense of oleic acid (18:1n-9). Both 18:3n-3 and 18:4n-3 were elongated and desaturated to eicosatetraenoic acid (20:4n-3), eicosapentaenoic acid (20:5n-3) and docosapentaenoic acid (22:5n-3), but not to docosahexaenoic acid (22:6n-3), and were incorporated into phospholipids and triacylglycerols. Over a 4-d period, the growth of NIH-3T3 cells was slightly stimulated in the presence of 18:3n-3 (20 micrograms/mL) but was strongly inhibited in the presence of 18:4n-3 at the same concentration. This inhibition may be caused by enhanced lipid peroxidation as a result of the high levels of 18:4n-3 present. |
| Databáze: | OpenAIRE |
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