Caffeine Inhibits Suicidal Erythrocyte Death
| Autor: | Michael Föller, Elisa Floride, Florian Lang, Markus Ritter |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Programmed cell death
medicine.medical_specialty Erythrocytes Physiology chemistry.chemical_element Phosphatidylserines Calcium Biology Cell membrane chemistry.chemical_compound Cytosol Chlorides Annexin Caffeine Internal medicine medicine Humans Cell Size Calcium metabolism Cell Death Phosphatidylserine Glucose Endocrinology medicine.anatomical_structure chemistry Biochemistry Apoptosis Isotonic Solutions |
| Zdroj: | Cellular Physiology and Biochemistry. 22:253-260 |
| ISSN: | 1421-9778 1015-8987 |
| DOI: | 10.1159/000149803 |
| Popis: | Eryptosis, the suicidal death of erythrocytes, is characterized by cell shrinkage and by cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surface. Eryptosis is triggered by several stress conditions including isotonic cell shrinkage (Cl(-) removal) and energy depletion (glucose removal). Both are effective through an increase in the cytosolic Ca(2+) concentration. Phosphatidylserine-exposing erythrocytes are cleared from circulating blood. Enhanced eryptosis thus leads to anemia. Accordingly, drugs interfering with eryptosis may prove useful in the treatment of anemia. The present study explored, whether caffeine interferes with eryptosis. Erythrocyte phosphatidylserine exposure was estimated from annexin V-binding, cell volume from forward scatter and cytosolic Ca(2+) activity from Fluo3 fluorescence. Under control conditions, eryptosis affected less than 5% of the erythrocytes and was not significantly modified by the presence of caffeine (50-500 microM). Glucose depletion (for 48 hours) significantly increased Fluo3 fluorescence and annexin V-binding and decreased forward scatter, effects partially reversed by caffeine (500 microM). Low Cl(-) solution (Cl(-) exchanged by gluconate for 48 hours) similarly increased annexin V-binding and decreased forward scatter, effects again reversed by caffeine (50-500 microM). In conclusion, caffeine inhibits Ca(2+) entry following glucose depletion and thus counteracts eryptosis during isotonic cell shrinkage and energy depletion. |
| Databáze: | OpenAIRE |
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