Controllable immobilization of naringinase on electrospun cellulose acetate nanofibers and their application to juice debittering
| Autor: | Yingfei Zhan, Weijuan Huang, Hongbing Deng, Xiaowen Shi, Yumin Du, Jiajia Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Alginates
Food Handling Limonin Nanofibers 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry chemistry.chemical_compound Hydrolysis Glucuronic Acid Multienzyme Complexes Structural Biology Specific surface area Polymer chemistry Cellulose Molecular Biology Naringin Hexuronic Acids beta-Glucosidase technology industry and agriculture General Medicine Enzymes Immobilized 021001 nanoscience & nanotechnology Cellulose acetate Electrospinning 0104 chemical sciences Fruit and Vegetable Juices chemistry Chemical engineering Taste Nanofiber Adsorption Naringinase 0210 nano-technology Porosity |
| Zdroj: | International Journal of Biological Macromolecules. 98:630-636 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.02.018 |
| Popis: | Electrospinning is a facile method to fabricate nanofibers, in terms of their high specific surface area and porous structure. Electrospun nanofibrous mats are excellent candidates for immobilization of enzymes. In this study, a simple route based on electrospinning and layer-by-layer (LBL) self-assembly processes has been developed to prepared naringinase/alginate multilayer coated electrospun cellulose acetate nanofibers. The content of immobilized naringinase could be tuned by adjusting the number of multilayers. XPS results indicated that naringinase was successfully immobilized on cellulose acetate nanofibers. SEM images showed the nanofibers maintain their sharp but became rougher after multilayer coating. Besides, the surface area of electrospun cellulose acetate nanofibers decreased and mesopores reduced. The major bitter components of grapefruit juice are naringin and limonin, naringin could be slightly removed by hydrolysis with naringinase and limonin might be removed by adsorption with cellulose acetate nanofibers. |
| Databáze: | OpenAIRE |
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