Amifostine use in radiation-induced kidney damage. Preclinical evaluation with scintigraphic and histopathologic parameters
| Autor: | Murat Caloglu, Sule Parlar, Rusen Cosar-Alas, Zafer Kocak, Vuslat Yurut-Caloglu, Mert Saynak, Semsi Altaner, Mevlüt Türe, M. Kaldir, Fusun Tokatli, Cem Uzal, Tevfik Fikret Cermik |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Tubular atrophy medicine.medical_treatment Premedication Intraperitoneal injection Urology Renal function Radiation-Protective Agents Scintigraphy Kidney Kidney Function Tests Amifostine Fibrosis Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Radiation Injuries Radionuclide Imaging Saline medicine.diagnostic_test business.industry medicine.disease Rats Endocrinology medicine.anatomical_structure Kidney Tubules Oncology Technetium Tc 99m Dimercaptosuccinic Acid Female business Injections Intraperitoneal medicine.drug |
| Zdroj: | Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]. 184(7) |
| ISSN: | 0179-7158 |
| Popis: | To assess the degree of protective effects of amifostine on kidney functions via semiquantitative static renal scintigraphy and histopathologic analysis. 30 female albino rats were divided into three equal groups as control (CL), radiotherapy alone (RT), and radiotherapy + amifostine (RT+AMI). The animals in the CL and RT groups were given phosphate-buffered saline, whereas the animals in the RT+AMI group received amifostine (200 mg/kg) by intraperitoneal injection 30 min before irradiation. RT and RT+AMI groups were irradiated with a single dose of 6 Gy using a 60Co unit at a source-skin distance of 80 cm to the whole right kidney. They were followed up for 6 months. CL, RT, and RT+AMI groups underwent static kidney scintigraphy at the beginning of the experiment and, again, on the day before sacrificing. Histopathologically, tubular atrophy and fibrosis of the kidney damage were evaluated. After irradiation, the median value of right kidney function was 48% (44–49%) and 50.5% (49%–52%) in RT and RT+AMI groups, respectively (p = 0.0002). Grade 1 kidney fibrosis was observed to be 60% in the RT group, while it was only 30% in the RT+AMI group. Grade 2 kidney fibrosis was 30% and 0% in the RT and RT+AMI group, respectively. Grade 1 tubular atrophy was 70% and 50% in the RT and RT+AMI group, respectively. Grade 2 tubular atrophy effect was the same in both groups (10%). Static kidney scintigraphy represents an objective and reproducible method to noninvasively investigate kidney function following irradiation. Amifostine produced a significant reduction in radiation-induced loss of renal function. |
| Databáze: | OpenAIRE |
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