Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon
| Autor: | Joanna M. Lukasiewicz, Rhys A. Farrer, Peter van Dam, Sarah M. Schmidt, Martijn Rep, Chiara Bertoldo |
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| Přispěvatelé: | Molecular Plant Pathology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Candidate gene Physiology Melon Population Plant Science Plant disease resistance Article Fungal Proteins 03 medical and health sciences Fusarium Cucumis melo Fusarium oxysporum Promoter Regions Genetic education Gene Phylogeny Disease Resistance Plant Diseases Plant Proteins Genetics Comparative genomics education.field_of_study Base Sequence biology Genetic Complementation Test Chromosome Mapping High-Throughput Nucleotide Sequencing food and beverages Genomics Sequence Analysis DNA biology.organism_classification Fusarium wilt 030104 developmental biology DNA Transposable Elements Genome Fungal |
| Zdroj: | New Phytologist, 209(1), 307-318. Wiley-Blackwell |
| ISSN: | 0028-646X |
| Popis: | Development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. Disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. These proteins secreted by pathogens are called 'avirulence' proteins. Their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. We have used genome sequencing of a set of strains of the melon wilt fungus Fusarium oxysporum f. sp. melonis (Fom), bioinformatics-based genome comparison and genetic transformation of the fungus to identify AVRFOM2, the gene that encodes the avirulence protein recognized by the melon Fom-2 gene. Both an unbiased and a candidate gene approach identified a single candidate for the AVRFOM2 gene. Genetic complementation of AVRFOM2 in three different race 2 isolates resulted in resistance of Fom-2-harbouring melon cultivars. AvrFom2 is a small, secreted protein with two cysteine residues and weak similarity to secreted proteins of other fungi.The identification of AVRFOM2 will not only be helpful to select melon cultivars to avoid melon Fusarium wilt, but also to monitor how quickly a Fom population can adapt to deployment of Fom-2-containing cultivars in the field. |
| Databáze: | OpenAIRE |
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