Effect of Solanum trilobatum on the antioxidant status during diethyl nitrosamine induced and phenobarbital promoted hepatocarcinogenesis in rat
| Autor: | Chennam Srinivasulu Shyamaladevi, G. Vani, M. Shahjahan |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment Glutathione reductase Pharmacology Solanum Toxicology Chemoprevention Thiobarbituric Acid Reactive Substances Antioxidants chemistry.chemical_compound Liver Neoplasms Experimental medicine TBARS Animals Anticarcinogenic Agents Glucose-6-phosphate dehydrogenase Diethylnitrosamine Solanum trilobatum chemistry.chemical_classification biology Plant Extracts Glutathione peroxidase General Medicine Glutathione biology.organism_classification Enzymes Rats Disease Models Animal Oxidative Stress Liver chemistry Biochemistry Catalase Phenobarbital Carcinogens biology.protein Lipid Peroxidation Precancerous Conditions |
| Zdroj: | Chemico-Biological Interactions. 156:113-123 |
| ISSN: | 0009-2797 |
| Popis: | Aim: The methanolic extract of Solanum trilobatum (ST) is cytotoxic and exerts an inhibitory effect on tumor growth and in the present study, its role on the antioxidant status of N-diethylnitrosamine (DEN) induced and phenobarbital (PB) promoted hepatocarcinogenesis was assessed. Methods: The protective role of ST on DEN induced and PB promoted hepatocarcinogenesis in Wistar rats was assessed from total nodular incidence, nodule multiplicity and volume of persistent nodules after an experimental period of 3 and 6 months following co-administration. The levels of thiobarbituric reactive substances (TBARS), glutathione (GSH) and activities of antioxidant enzymes were assessed in the haemolysate and liver of experimental animals to evaluate the antioxidant status. Results: In DEN + PB + ST animals, the nodular incidence, multiplicity and volume reduced significantly compared to DEN + PB treated animals. In DEN + PB animals, the levels of TBARS increased significantly, whereas the levels of GSH and the activities of antioxidant enzymes-superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and glucose 6 phosphate dehydrogenase showed significant alterations compared to control both in the haemolysate and liver. However, in DEN + PB + ST animals, the levels of TBARS decreased significantly and the levels of GSH increased with favorable alterations in the activities of antioxidant enzymes in both the haemolysate and liver. Conclusion: The present results suggest that ST exerts its chemopreventive effects by modulating the antioxidant status during DEN induced hepatocarcinogenesis. |
| Databáze: | OpenAIRE |
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