Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus

Autor: Yu-Jung Won, Soon-Young Paik, Seung-Won Park, Lae Hyung Kang, Jae Woong Lee, Sung-Geun Lee
Rok vydání: 2020
Předmět:
Phlebovirus
Severe Fever with Thrombocytopenia Syndrome
Gastrointestinal Diseases
reverse transcription loop-mediated isothermal amplification (LAMP)
Loop-mediated isothermal amplification
Sensitivity and Specificity
Applied Microbiology and Biotechnology
Microbiology
Article
molecular diagnostics
03 medical and health sciences
Leukocytopenia
Republic of Korea
Humans
Medicine
DNA Primers
virus detection
030304 developmental biology
0303 health sciences
biology
030306 microbiology
business.industry
Mortality rate
severe fever with thrombocytopenia syndrome (STFS)
RNA virus
General Medicine
medicine.disease
Molecular diagnostics
biology.organism_classification
Virology
Reverse transcriptase
Severe fever with thrombocytopenia syndrome
Molecular Diagnostic Techniques
RNA
Viral
business
Nucleic Acid Amplification Techniques
Severe fever with thrombocytopenia syndrome virus
Zdroj: Journal of Microbiology (Seoul, Korea)
ISSN: 1976-3794
1225-8873
DOI: 10.1007/s12275-020-0109-1
Popis: Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80–100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172 cases reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RT-LAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS.
Databáze: OpenAIRE
Externí odkaz: