The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris
| Autor: | Jürgen Köhler, Sebastian R. Beyer, Lars Müller, Richard J. Cogdell, G. Matthias Ullmann, June Southall |
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| Rok vydání: | 2014 |
| Předmět: |
Photosynthetic reaction centre
education.field_of_study biology Chemistry Population Light-Harvesting Protein Complexes biology.organism_classification Surfaces Coatings and Films Rhodopseudomonas Spectrometry Fluorescence Orders of magnitude (time) Bacterial Proteins Master equation Materials Chemistry Computer Simulation Physical and Theoretical Chemistry Atomic physics Time-resolved spectroscopy Rhodopseudomonas palustris Spectroscopy education Excitation |
| Zdroj: | The journal of physical chemistry. B. 119(4) |
| ISSN: | 1520-5207 |
| Popis: | We studied the time-resolved fluorescence of isolated RC-LH1 complexes from Rhodopseudomonas palustris as a function of the photon fluence and the repetition rate of the excitation laser. Both parameters were varied systematically over 3 orders of magnitude. On the basis of a microstate description we developed a quantitative model for RC-LH1 and obtained very good agreement between experiments and elaborate simulations based on a global master equation approach. The model allows us to predict the relative population of RC-LH1 complexes with the special pair in the neutral state or in the oxidized state P(+) and those complexes that lack a reaction center. |
| Databáze: | OpenAIRE |
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