Jasmonate-Elicited Stress Induces Metabolic Change in the Leaves of Leucaena leucocephala

Autor: Amy G. W. Gong, Yunfei Yuan, Yingchao Xu, Shuangyan Chen, Zhenru Tao, Yu Jin, Zhong-Yu Zhou, Karl Wah Keung Tsim, Tina T. X. Dong
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Magnetic Resonance Spectroscopy
principal component analysis
Metabolite
Pharmaceutical Science
Cyclopentanes
Environment
Leucaena leucocephala
01 natural sciences
Article
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
lcsh:Organic chemistry
Stress
Physiological
elicitors
Drug Discovery
Metabolome
Metabolomics
Herbivory
Oxylipins
Jasmonate
Physical and Theoretical Chemistry
Threonine
biology
Jasmonic acid
Organic Chemistry
Fabaceae
biology.organism_classification
NMR
partial least squares discriminant analysis
environmental stress
Carbon
Lactic acid
Plant Leaves
030104 developmental biology
chemistry
Biochemistry
Chemistry (miscellaneous)
Glycine
Molecular Medicine
Protons
Energy Metabolism
010606 plant biology & botany
Zdroj: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Molecules, Vol 23, Iss 2, p 188 (2018)
Molecules; Volume 23; Issue 2; Pages: 188
ISSN: 1420-3049
DOI: 10.3390/molecules23020188
Popis: The plant Leucaena leucocephala was exposed to four jasmonate elicitors, i.e., jasmonic acid (JA), methyl jasmonic acid (MeJA), jasmonoyl-l-isoleucine (JA-Ile) and 6-ethyl indanoyl glycine conjugate (2-[(6-ethyl-1-oxo-indane-4-carbonyl)-amino]-acetic acid methyl ester) (CGM). The treatment was to mimic the herbivores and wounding stresses. By using NMR spectroscopy along with chemometric analysis, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), the changes of metabolites in the leaves of L. leucocephala were determined under the stress as induced by the four elicitors. The challenge of JA-Ile caused an accumulation of lactic acid (6), β-glucose (10), alanine (12), threonine (13), steroids (18), 3,4-dihydroxypyridine (19) and an unidentified compound 20. The chemometric analysis of the PCA and PLS-DA models indicated that the alternation of metabolites triggered by JA, MeJA, and CGM treatments were very minimum. In contrast, the treatment by JA-Ile could induce the most significant metabolic changes in the leaves. Moreover, there was very minimal new metabolite being detected in responding to the jasmonate-induced stresses. The results showed some metabolite concentrations changed after application of the elicitors, which may be related to a high level of tolerance to stress conditions as well as the strong ecological suitability of L. leucocephala.
Databáze: OpenAIRE
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