Effects of a variety of cytokines and inducing agents on vascular permeability factor mRNA levels in U937 cells
| Autor: | Daniel T. Connolly, Gregory J. Dolecki |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Angiogenesis medicine.medical_treatment Biophysics Endothelial Growth Factors Biology Biochemistry Polymerase Chain Reaction Cell Line Transforming Growth Factor beta Internal medicine medicine Humans RNA Messenger RNA Neoplasm Molecular Biology Lymphokines U937 cell Vascular Endothelial Growth Factors Monocyte Growth factor Cell Differentiation Cell Biology Blotting Northern Clone Cells Endothelial stem cell Kinetics medicine.anatomical_structure Endocrinology Cytokine Cell culture biology.protein Cytokines RNA Tetradecanoylphorbol Acetate DNA Probes Poly A Platelet-derived growth factor receptor |
| Zdroj: | Biochemical and biophysical research communications. 180(2) |
| ISSN: | 0006-291X |
| Popis: | Summary Vascular permeability factor (VPF) is an approximately 40-kDa disulfide-linked dimeric glycoprotein that is active in increasing blood vessel permeability, endothelial cell growth and angiogenesis. Little is known about VPF gene regulation. In this study, we investigated the effects of a variety of cytokines and inducing agents on VPF mRNA levels in the monocyte-like U937 cell line. Transforming growth factor-beta 1 caused a 1.8-fold increase in VPF mRNA levels after 4 hours, followed by a decline to basal levels by 18 hours. Phorbol 12-myristate 13-acetate, a potent inducer of the differentiation of U937 cells, caused a 12.5-fold increase in VPF mRNA levels at 24 hours, coinciding with the differentiation of these monocyte-like cells into macrophage-like cells. |
| Databáze: | OpenAIRE |
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