Direct Determination of Serum Glycyrrhetic Acid by a Monoclonal Antibody-Based Inhibition Elisa Using Ibuprofen for Releasing Serum Albumin-Bound Glycyrrhetic Acid
| Autor: | Michinao Mizugaki, Yoshihisa Tomioka, Masataka Hayasaka, Kunihiko Itoh, Shunji Ishiwata, Masahiro Niizeki, Seishiro Nozaki |
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| Rok vydání: | 1996 |
| Předmět: |
Immunology
Serum albumin Enzyme-Linked Immunosorbent Assay Ibuprofen Binding Competitive chemistry.chemical_compound Pharmacokinetics medicine Humans Enoxolone Serum Albumin Pharmacology Chloroform Chromatography Dose-Response Relationship Drug biology Chemistry Antibodies Monoclonal Human serum albumin Dose–response relationship biology.protein Glycyrrhetinic Acid Antibody Protein Binding medicine.drug |
| Zdroj: | Journal of Immunoassay. 17:343-352 |
| ISSN: | 0197-1522 |
| DOI: | 10.1080/01971529608005797 |
| Popis: | We found that ibuprofen (IBU) had a potential for releasing serum albumin-bound glycyrrhetic acid (GA). Based on this observation, IBU was used to pretreat samples for the determination of serum GA levels by an inhibition ELISA. This method, termed IBU method was evaluated by the recovery of GA from human serum albumin (HSA) or normal human serum (NHS) that contained the exogeneously added GA (37-1000 ng/ml). The mean recovery of GA from HSA and NHS samples treated with IBU were 104.7 and 105.2%, respectively, whereas those without IBU pretreatment were 2.8 and 10.7%, respectively. Comparison of IBU method and chloroform extraction method revealed that the GA content of serum samples pretreated by each method were almost the same. These results suggest that IBU method is useful as a serum processing procedure for the determination of serum GA levels by an inhibition ELISA. |
| Databáze: | OpenAIRE |
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