| Autor: |
Chen, Wentao, Xu, Ziye, You, Wenjing, Zhou, Yanbing, Wang, Liyi, Huang, Yuqin, Shan, Tizhong |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22615613 |
| Popis: |
Additional file 5: Fig. S4. Short-term cold exposure induces transcriptome programs alterations. (A-C) Gene set enrichment analysis showing significant enrichment in macroautophagy, autophagy, and mitophagy from control and cold-treated mice. (D) Heatmap showing the differentially expressed genes related to autophagy (padj < 0.05 & Abs (Log2 fold changes) > 1) in TA muscles from control and cold-treated mice. Red and blue indicate upregulated differential and downregulated differential expression genes, respectively (n = 4). (E) Western blot of BNIP3 and LC3B proteins expression in TA muscles from RT and cold-treated mice, GAPDH as loading control. (F) mRNA expression of the autophagy (Atg5 and Atg7) and mitophagy (Pink1, Park2, and Bnip3) genes in TA muscles from RT and cold-treated mice (n = 4). (G-H) Western blot and quantitative analysis of mitochondrial fractions showing oxidative phosphorylation protein in TA muscles from RT and cold-treated mice, GAPDH as loading control. Error bars represent s.e.m.* P < 0.05, ** P < 0.01, *** P < 0.001, two-tailed Student’s t-test. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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